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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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商品描述
Background:
LSD1isachromatin-modifyingenzymethatspecificallyremovesmethylgroupsfrommono-anddimethylatedLysofhistoneH3.LSD1isacriticalcomponentoftranscriptionalregulationviaepigenetichistonemodificationsandisthereforeapotentialtargetfordrugdevelopment.
Description:
TheLSD1AssayKitisdesignedtomeasureLSD1activityofpurifiedLSD1enzymescontainingLSD1usingfluorimetry.TheassayisbasedontheanalysisofthehydrogenperoxideproducedduringthedemethylationreactionmediatedbyLSD1.ThekeytotheLSD1AssayKitisthemethylatedpeptidesubstrate.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforLSD1reactions.First,asamplecontainingLSD1isincubatedinareactionmixturewiththemethylatedpeptidesubstrate.Second,peroxidaseandAmplexRedreagentareaddedandfluorescence(λex=530±13nm,λem=590±18nm)ismeasuredusingaplatereader.
UniProt
O60341
Synonym(s):
KDM1A,AOF2,LSD1,BHC110,LSD
SuppliedAs:
TheLSD1AssayKitisprovidedinaconvenient96-wellformat,withpurifiedLSD1,AmplexRedreagent,peroxidase,andLSD1assaybufferfor100enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.FornerisF,etal.JBiolChem2006;281(46):35289-95.
2.ZhouM,etal.AnalBiochem1997;253(2):162-8.
ApplicationReference(s):
1. HistoneH3peptidesincorporatingmodifiedlysineresiduesaslysine-specificdemethylase1inhibitors(2017)
2. Structure-activitystudyfor(bis)ureidopropyl-and(bis)thioureidopropyldiamineLSD1inhibitorswith3-5-3and3-6-3carbonbackbonearchitectures(2015)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Demethylase
ProductType:
AssayKit
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