Phospholipid Membranes. Thelipids palmitoyloleoylphos- phocholine (POPC, Avanti Polar Lipids) andN-(6-tetrameth - 百度学术 摘要： Inrecent years observations atthelevel of individual atomsandmolecules becamepossible bymicros- copy andspectroscopy. Imaging ofsingle fluorescence mole- cules hasbeenachieved buthassofarbeenrestricted to molecules intheimmobile state. Hereweprovide methodology forvisualization ofthemotion ofindividual fluorescent mol- ecules. Itisapplied toimaging ofthediffusional path ofsingle molecules inaphospholipid membranebyusing phospholip- idscarrying onerhodamine dye molecule. Forthis method- ology, fluorescence microscopy wascarried toasensitivity so thatsingle fluorescent molecules illuminated foronly 5ms wereresolvable atasignal/noise ratio of28.Repeated illu- minations permitted direct observation ofthediffusional motion ofindividual molecules withapositional accuracy of 30nm.Suchcapability hasfascinating potentials inbio- science-for example, tocorrelate biological functions ofcell membranes withmovements, spatial organization, andstoi- chiometries ofindividual components. 展开 我们已与文献出版商建立了直接购买合作。 你可以通过身份认证进行实名认证，认证成功后本次下载的费用将由您所在的图书馆支付 您可以直接购买此文献，1~5分钟即可下载全文，部分资源由于网络原因可能需要更长时间，请您耐心等待哦~

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