ZeptoMetrix成立于1999年,是一家为药物研究行业提供诊断产品和服务的的私人控股公司。zeptometrix公司(ZMC)是一个传染病诊断行业先进者和创新者,为全球的客户提供值得信赖的产品和服务。zeptometrix是一个完全集成的生物技术公司的产品支持的研发的所有阶段;发展,验证,制和商业化的诊断测试。zeptometrix不断开拓创新,致力为生命科学行业带来更多产品与服务。
Catalog #: 0801198
Bovine IgG ELISA
Preparation of Bovine IgG Standards
Tube
Number
Concentration of
Bovine IgG
Volume of Bovine IgG
Standard
Volume of
Assay Diluent
1
125 ng/mL
1000 ml
0 ml
2
62.5 ng/mL
500 ml of #1
500 ml
3
31.25 ng/mL
500 ml of #2
500 ml
4
15.6 ng/mL
500 ml of #3
500 ml
5
7.8 ng/mL
500 ml of #4
500 ml
6
0 ng/mL
0 ml
500 ml
试验过程
使用前允许所有试剂达到室温。用于制备标准和样品的标号试管。如果不使用整个96井板,则取下剩余的井板。 从板框和放置到重新密封塑料袋与干燥剂。密封袋并存放在2-8 C。
步骤1:将每条标签贴在其末端标签上,以确保在检测过程中这些条带与板框分离时的标识。
步骤2:将标准1-6的200ml移入重复井.
步骤3:每个样品的移液管200 L成重复的威尔斯。
第四步:用盖板盖上盖板,在37 C下孵育30分钟。
第五步:抽取每口井的内容物,用1x板冲洗缓冲液冲洗4次。清洗时,用1倍平板冲洗缓冲液的300ml注满井,然后抽吸。执行4次填充/吸气循环 s.在后的清洗周期之后,仔细地在吸水性纸巾的垫子上仔细地敲打盘子,彻底地擦拭盘子。继续,直到没有观察到可见的液滴板洗缓冲液。
步骤6:将检测抗体的100m1移入各标准标本中。
不要将检测器抗体添加到底物空白井中。
步骤7:用盖板盖上盖板,在37 C下孵育30分钟。
步骤8:如步骤6所述,用板洗缓冲器冲洗4次。
步骤9:将基材的100m1移入每口井,包括基板空白井。
步骤10:室温下将盘子孵育30分钟。含有牛IgG的井中会出现蓝色。
步骤11:将100m1的止住溶液注入每口井。将发生从蓝色到黄色的颜色变化。
步骤12:在15分钟内,用微滴度平板阅读器读取450 nm处每口井的光密度。
试样稀释
牛初乳通常含有50~80 mg/mL的IgG抗体。1:1,000,000牛初乳稀释在试验稀释剂中被推荐用于初步测试。
牛乳通常含有0.5mg/mL的IgG抗体。建议在试验稀释液中稀释1:10,000的牛乳进行初步测试。
牛血清通常含有25-30mg/mL的IgG抗体。建议在试验稀释剂中稀释1:500,000牛血清进行初步测试。
程序流程图
制备试剂稀释剂
吸管标本和标准
37℃孵育30分钟
制水板
移液管检测器抗体
37℃孵育30分钟
制水板
移液管底液
室温孵育30分钟
添加停止溶液并在450 nm处读取
贮存
在2-8℃下储存所有试剂盒试剂。
不要冻结。
未使用的微量培养板应保存在带有干燥剂的密封袋中,以使暴露于水分降至低。
储存和处理的所有试剂均为ST 至少可以在包盒标签上打印的过期日期之前。
货号
品名
目录价
规格
品牌
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Streptococcus Agalactiae 2603V - R, DNA
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0801562DNA-10UG
Bacillus Subtilis Z094, DNA
7001
10 ug
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0801563DNA-10UG
Citrobacter FreundII Z064, DNA
7001
10 ug
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0801569DNA-10UG
Stenotrophomonas Maltophilia Z074, DNA
7001
10 ug
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0801536DNA-1UG
Candida Krusei Z009, DNA
4524
1 ug
ZeptoMetrix
0801537DNA-1UG
Candida Parapsilosis Z011, DNA
4524
1 ug
ZeptoMetrix
0801535DNA-1UG
Candida Glabrata Z007, DNA
4524
1 ug
ZeptoMetrix
0801519DNA-10UG
Pseudomonas Aeruginosa clinical isolate, DNA
7001
10 ug
ZeptoMetrix
0801534DNA-10UG
Listeria Monocytogenes serotype 1 - 2b, DNA
7001
10 ug
ZeptoMetrix
0801518DNA-10UG
Enterobacter Aerogenes Z052, DNA
7001
10 ug
ZeptoMetrix
0801511DNA-10UG
Neisseria Meningitidis serogroup A, DNA
7001
10 ug
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0801506DNA-10UG
Klebsiella Pneumoniae Z026, DNA
7001
10 ug
ZeptoMetrix
0801504DNA-1UG
Candida Albicans Z006, DNA
4524
1 ug
ZeptoMetrix
0801597DNA-10UG
Acinetobacter BaumannII 307-0294, DNA
7001
10 ug
ZeptoMetrix
0801579DNA-10UG
Mycoplasma Pneumoniae M129, DNA
7001
10 ug
ZeptoMetrix
0801679DNA-10UG
Haemophilus Influenzae type b; Eagan, DNA
7001
10 ug
ZeptoMetrix
0801676DNA-1UG
Histoplasma capsulatum Recombinant, DNA
7001
1 ug
ZeptoMetrix
0801677DNA-1UG
Coccidioides Immitis Recombinant, DNA
7001
1 ug
ZeptoMetrix
0801675DNA-10UG
Staphylococcus Aureus MSSA; mecA-deficient, DNA
7001
10 ug
ZeptoMetrix
0801674DNA-10UG
Yersinia Enterocolitica clinical isolate, DNA
7001
10 ug
ZeptoMetrix
0801651DNA-10UG
Staphylococcus Epidermidis MRSE; RP62A, DNA
7001
10 ug
ZeptoMetrix
0801650DNA-10UG
Campylobacter Jejuni clinical isolate, DNA
7001
10 ug
ZeptoMetrix
0801638DNA-10UG
Staphylococcus Aureus MRSA; COL, DNA
7001
10 ug
ZeptoMetrix
0801627DNA-10UG
Shigella Sonnei Z004, DNA
7001
10 ug
ZeptoMetrix
0801624DNA-10UG
Escherichia Coli ETEC; ST+, LT+ DNA
2730
10 ug
ZeptoMetrix
0801622DNA-10UG
Escherichia Coli O157:H7; EDL933, DNA
2730
10 ug
ZeptoMetrix
0801603DNA-1UG
Candida Lusitaniae Z010, DNA
4524
1 ug
ZeptoMetrix
0801619DNA-1UG
Clostridium Difficile NAP1, DNA
7001
1 ug
ZeptoMetrix
0801601DNA-1UG
Aspergillus Terreus Z016, DNA
4524
1 ug
ZeptoMetrix
0801602DNA-1UG
Candida GuilliermondII Z008, DNA
4524
1 ug
ZeptoMetrix
0801598DNA-1UG
Aspergillus Flavus Z013, DNA
4524
1 ug
ZeptoMetrix
0801680DNA-10UG
Haemophilus Influenzae type b; MinnA, DNA
7001
10 ug
ZeptoMetrix
0801482DNA-10UG
Neisseria Gonorrhoeae type strain, DNA
7001
10 ug
ZeptoMetrix
0801439DNA-10UG
Streptococcus Pneumoniae Z022; 19F (Danish), DNA
7001
10 ug
ZeptoMetrix
801223
Goat IgG ELISA
4875
96 det.
ZeptoMetrix
801010
Chicken IgY ELISA
4875
96 det.
ZeptoMetrix
801198
Bovine IgG ELISA
6689
96 det.
ZeptoMetrix
801188
ELISA Construction System
5343
5 plates
ZeptoMetrix
0801185L
ZeptoBlock
3842
1 L
ZeptoMetrix
801185
ZeptoBlock
1697
120 ml
ZeptoMetrix
0801184L
ZeptoBind
3842
1 L
ZeptoMetrix
0801184
ZeptoBind
839
60 ml
ZeptoMetrix
0801179L
ZeptoCoat
3842
1 L
ZeptoMetrix
0801179
ZeptoCoat
839
60 ml
ZeptoMetrix
0802001
rhIL-2 Recominant Human Interleukin-2
5850
100 ug
ZeptoMetrix
0801060
10x Plate Wash Buffer
293
125 ml
ZeptoMetrix
0801114
rhIL-2 Recominant Human Interleukin-2 (20.000 units)
1463
1.1 ml
ZeptoMetrix
0801017
TCGF (Natural T-Cell Growth Factor, IL-2)
4700
50 ml
ZeptoMetrix
0801195
ZeptoGel
5207
500 ml
ZeptoMetrix
0801002
HIV-1 p24 Antigen ELISA 2.0 Kit
9341
96 det.
ZeptoMetrix
0801221
Horse IgG ELISA
4875
96 det.
ZeptoMetrix
0801008
HIV-1 p24 Antigen ELISA 2.0 Kit
38805
480 det.
ZeptoMetrix
0801437DNA-10UG
Salmonella Enterica Typhimurium Z005, DNA
7001
10 ug
ZeptoMetrix
0801803P
Cryptococcus Neoformans Serotype A, antigen
1092
1.0 ml
ZeptoMetrix
0801202
Rabbit IgG ELISA
6689
96 det.
ZeptoMetrix
0801009
Rat IgG ELISA
4875
96 det.
ZeptoMetrix
0801181
Mouse IgM ELISA
6689
96 det.
ZeptoMetrix
0801201
SIV p27 Antigen ELISA Kit
49764
480 det.
ZeptoMetrix
0801200
HIV-1 p24 Antigen ELISA Kit
38805
480 det.
ZeptoMetrix
0801197
Human IgA ELISA
6689
96 det.
ZeptoMetrix
0801183
Human IgM ELISA
6689
96 det.
ZeptoMetrix
0801182
Human IgG ELISA
6689
96 det.
ZeptoMetrix
0801169
SIV p27 Antigen ELISA Kit
11993
96 det.
ZeptoMetrix
0801137
HIV-1 p24 Extended Range Kit
1346
kit
ZeptoMetrix
0801116
HTLV p19 Antigen ELISA Kit
11993
96 det.
ZeptoMetrix
0801111
HIV-1 p24 Antigen ELISA Kit
9341
96 det.
ZeptoMetrix
0801096
HIV-1 p24 ICx - CRx Kit
1853
100 det.
ZeptoMetrix
0801180
Mouse IgG ELISA
6689
96 det.
ZeptoMetrix
0801693DNA-10UG
Enterococcus Faecalis VRE, DNA
7001
10 ug
ZeptoMetrix
0801084
Anti-HTLV-I gp46 Clone 67 - 5.5.13.1
4388
100 ug
ZeptoMetrix
0801119
Anti-HTLV-II gp46 Clone 73 - 4.9.8
30732
1 mg
ZeptoMetrix
0801120
Anti-HTLV-II p24 Clone 75 - 4.21.11
30732
1 mg
ZeptoMetrix
0801012
Anti-PON1 Clone KRJ1
4388
100 ug
ZeptoMetrix
0801014
Anti-PON1 Clone KRJ2
4388
100 ug
ZeptoMetrix
0801095
Anti-PON1 Clone KRJ1
30732
1 mg
ZeptoMetrix
0801097
Anti-PON1 Clone KRJ2
30732
1 mg
ZeptoMetrix
0801025
Anti-Zika NS1 Clone 1D12
4388
100 ug
ZeptoMetrix
0801032CFHI
HIV Type 1 Strain: IIIB - CFHI
6045
1.0 ml
ZeptoMetrix
0801033CFHI
Human T-Lymphotropic Virus Type I (HTLV-I) - CFHI
6045
1.0 ml
ZeptoMetrix
0801113
Anti-HTLV-II p19 Clone 78 - 6.18.07
30732
1 mg
ZeptoMetrix
0801093
Anti-HTLV-II p19 Clone 78 - 6.18.07
4388
100 ug
ZeptoMetrix
0801094
Anti-HTLV-I gp46 Clone 65 - 6C2.2.34
30732
1 mg
ZeptoMetrix
0801107
Anti-HTLV-I p19 Clone TP-7
30732
1 mg
ZeptoMetrix
0801108
Anti-HTLV-I p19 Clone 45 - 6.11.1.3
30732
1 mg
ZeptoMetrix
0801110
Anti-HTLV-I p24 Clone 46 - 3.24.4
30732
1 mg
ZeptoMetrix
0801127
Anti-HTLV-I gp46 Clone 67 - 5.5.13.1
30732
1 mg
ZeptoMetrix
0801118
Anti-HTLV-I gp46 Clone 68 - 4.11.21
30732
1 mg
ZeptoMetrix
0802004
Anti-HTLV-I gp46 Clone 65 - 6C2.2.34
4388
100 ug
ZeptoMetrix
0801086
Anti-HTLV-II gp46 Clone 73 - 4.9.8
4388
100 ug
ZeptoMetrix
0801087
Anti-HTLV-II p24 Clone 75 - 4.21.11
4388
100 ug
ZeptoMetrix
0801032
Human Immunodeficiency Virus Type 1 (HIV-1) Strain: IIIB Lysate
27593
1 mg
ZeptoMetrix
0801033
Human T-Lymphotropic Virus Type I (HTLV-I) Lysate
31181
1 mg
ZeptoMetrix
0801216
Dinucleotide Standards ThymineGlycolCytidineDimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801217
Dinucleotide Standards ThymineGlycolAdenineDimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801218
Nucleoside Standards 8-Oxo-dG
1287
1 mg
ZeptoMetrix
0801219
Nucleoside Standards 8-Oxo-dA
1365
1 mg
ZeptoMetrix
0801384
PON Standards
5265
600 ul
ZeptoMetrix
0801500
Simian Immunodeficiency Virus (SIV) Kit (10 Strip)
9360
10 det.
ZeptoMetrix
0801501
Simian Immunodeficiency Virus (SIV) Kit (30 Strip)
22854
30 det.
ZeptoMetrix
0801502
Simian Immunodeficiency Virus (SIV) Strips (10/tube)
5343
10 strips
ZeptoMetrix
0801021
Zika (PRVABC59) Strips (10/tube)
5343
10 strips
ZeptoMetrix
0801215
Dinucleotide Standards ThymineGlycolThymineDimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801214
Dinucleotide Standards ThymineGlycolGuanineDimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801808
Scedosporium prolificans Z083
8775
1.0 ml
ZeptoMetrix
0801192
TBARS Assay kit
7995
160 det.
ZeptoMetrix
0801199
Arylesterase - Paraoxonase Assay Kit
7995
200 tests
ZeptoMetrix
0805002
Total Glutathione Peroxidase Assay Kit
7625
100 tests
ZeptoMetrix
0805004
Glutathione Reductase Assay Kit
10257
100 tests
ZeptoMetrix
0801210
Dinucleotide Standards Formamido Guanine Dimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801211
Dinucleotide Standards Formamido Cytidine Dimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801212
Dinucleotide Standards Formamido Thymine Dimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801213
Dinucleotide Standards Formamido Adenine Dimer
1287
50 mg at 1.0 OD
ZeptoMetrix
0801022
Zika (MR-766) Strips (10/tube)
5343
10 strips
ZeptoMetrix
0801085
Anti-HTLV-I gp46 Clone 68 - 4.11.21
4388
100 ug
ZeptoMetrix
0801698DNA-1UG
Pneumocystis Jiroveci Recombinant W303-Pji, Genomic DNA
7001
10 ug
ZeptoMetrix
0801765DNA-1UG
Scedosporium Apiospermum Z085, DNA
4524
1 ug
ZeptoMetrix
0801770DNA-1UG
Fusarium Oxysporum Z084, DNA
4524
1 ug
ZeptoMetrix
0801802DNA-1UG
Tremella Fuciformis Recombinant, DNA
7001
1 ug
ZeptoMetrix
0801806DNA-1UG
Fusarium solani Z081, DNA
4524
1 ug
ZeptoMetrix
0801808DNA-1UG
Scedosporium Prolificans Z083, DNA
4524
1 ug
ZeptoMetrix
0801827DNA-1UG
Aspergillus Niger Z105, DNA
4524
1 ug
ZeptoMetrix
0801834DNA-1UG
Rhizopus oryzae Z106, DNA
4524
1 ug
ZeptoMetrix
0801899DNA-10UG
Plesiomonas shigelloides Z130, DNA
7001
10 ug
ZeptoMetrix
0801901DNA-10UG
Vibrio Cholerae Z132 (toxigenic)
7001
10 ug
ZeptoMetrix
0801761DNA-1UG
Mucor Indicus Z079, DNA
4524
1 ug
ZeptoMetrix
0801759DNA-1UG
Cunninghamella bertholletiae Z080, DNA
4524
1 ug
ZeptoMetrix
0801700DNA-1UG
Cryptosporidium Parvum-S. cerevisiae Recombinant, DNA
7001
1.0 ml
ZeptoMetrix
0801716DNA-1UG
Aspergillus Fumigatus Z014, DNA
4524
1 ug
ZeptoMetrix
0801723DNA-10UG
Serratia Marcescens Z053, DNA
7001
10 ug
ZeptoMetrix
0801697DNA-1UG
Blastomyces Dermatitidis Recombinant W303-Bde, Genomic DNA
7001
1 ug
ZeptoMetrix
0801736DNA-10UG
Haemophilus ducreyi class I, DNA
7001
10 ug
ZeptoMetrix
0801743DNA-10UG
Bacteroides Thetaiotaomicron Z037 DNA
7001
10 ug
ZeptoMetrix
0801745DNA-10UG
Citrobacter Koseri Z039, DNA
7001
10 ug
ZeptoMetrix
0801747DNA-10UG
Escherichia coli O111:NM, DNA
7001
10 ug
ZeptoMetrix
0801755DNA-1UG
Rhizopus Microsporus Z056, DNA
4524
1 ug
ZeptoMetrix
0801004
Anti-HIV-l p24 Clone 38 - 8.7.47
4388
100 ug
ZeptoMetrix
0801005
Anti-HIV-l p17 Clone 32 - 5.8.42
4388
100 ug
ZeptoMetrix
0801124
Anti-HIV-l p24 Clone 32 - 5.17.76
30732
1 mg
ZeptoMetrix
0801125
Anti-HIV-l p24 Clone 38 - 8.7.47
30732
1 mg
ZeptoMetrix
0801126
Anti-HIV-l p24 Clone 39 - 5.1.23
30732
1 mg
ZeptoMetrix
0801128
Anti-HIV-l RT Clone 39 - 4.12.2
30732
1 mg
ZeptoMetrix
0801136
Anti-HIV-l p24 Clone 39 - 5.4A
30732
1 mg
ZeptoMetrix
0801003
Anti-HTLV-I p19 Clone TP-7
4388
100 ug
ZeptoMetrix
0802014
Anti-HIV-l p24 Clone 39 - 6.14
30732
1 mg
ZeptoMetrix
0801018
Anti-HTLV-I p24 Clone 46 - 3.24.4
4388
100 ug
ZeptoMetrix
0801082
Anti-HTLV-I p19 Clone 45 - 6.11.1.3
4388
100 ug
ZeptoMetrix
0801123
Anti-HIV-l p17 Clone 32 - 5.8.42
30732
1 mg
ZeptoMetrix
0801122
Anti-HIV-l p17 Clone 2D11
30732
1 mg
ZeptoMetrix
0801121
Anti-HIV-l gp41 Clone 10E9
30732
1 mg
ZeptoMetrix
0801006
Anti-HIV-l gp41 Clone 10E9
4388
100 ug
ZeptoMetrix
0801007
Anti-HIV-l RT Clone 39 - 4.12.2
4388
100 ug
ZeptoMetrix
0801076
Anti-HIV-l p17 Clone 2D11
4388
100 ug
ZeptoMetrix
0801077
Anti-HIV-l p17 Clone 32 - 1.24.89
4388
100 ug
ZeptoMetrix
0801078
Anti-HIV-l p24 Clone 32 - 5.17.76
4388
100 ug
ZeptoMetrix
0801079
Anti-HIV-l p24 Clone 39 - 5.1.23
4388
100 ug
ZeptoMetrix
0801080
Anti-HIV-l p24 Clone 39 - 5.4A
4388
100 ug
ZeptoMetrix
0801081
Anti-HIV-l p24 Clone 39 - 6.14
4388
100 ug
ZeptoMetrix
0801115
Anti-HIV-l p17 Clone 32 - 1.24.89
30732
1 mg
ZeptoMetrix
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