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商品描述
ProductDescription
CatalogueNumber: | AN00006 |
ProductName: | anti-4EBP1antibody |
Form: | liquid |
Host: | Mouse |
Clonity: | monoclonal |
Isotope: | IgG2b |
Storage: | PBSwith0.02%sodiumazideand50%glycerolpH7.3,-20â&BDquo;ƒfor24months(Avoidrepeatedfreeze/thawcycles.) |
Background: | Eukaryotictranslationinitiationfactor4E-bindingprotein1(4EBP1),isamemberof4EBPsfamily,whichregulatethetranslationofasubsetofmRNAbycompetingwitheIF4GforbindingtoeIF4E,thuspreventingtheassemblyoftheeIF4Fcomplex.TheeIF4Ffacilitatestherecruitmentofothertranslationinitiationfactorstoformthecomplexandtheninitiatescap-dependenttranslation.4EBP1alsomediatestheregulationofproteintranslationbygrowthfactors,hormonesandotherstimulithatsignalthroughtheMAPkinaseandmTORC1pathways.Therearethreeformsof4EBP1,alpha,betaandgamma. |
Immunogen: | eukaryotictranslationinitiationfactor4Ebindingprotein1 |
Synonyms: | 4EBP1,4EBP1,BP1,eIF4Ebindingprotein1,EIF4EBP1,PHASI |
CalculatedMW: | 12kDa |
Specificity: | Human,Mouse |
TestedApplication: | ELISA,WB,IHC |
RecommendedDilution: | WB:1:1000-1:10000;IHC:1:20-1:200;IF:1:20-1:200 |
IHCDilution: | Immunohistochemistryofparaffin-embeddedhumanpancreasslideusing4EBP1Antibodyatdilutionof1:50 |
WBDilution: | K-562cellsweresubjectedtoSDSPAGEfollowedbywesternblotwith4EBP1antibodyatdilutionof1:2000 |