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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Bevacizumab (Avastin®) is a recombinant humanized monoclonal antibody that blocks angiogenesis by inhibiting vascular endothelial growth factor A (VEGFA). VEGF-A stimulates angiogenesis in a variety of diseases, including cancer. Bevacizumab was the first clinically available angiogenesis inhibitor in the United States.
Sample Type
Serum and plasma
Intended Use
The Bevacizumab PK BioAssay™ ELISA kit is designed to measure Bevacizumab in serum or plasma with high specificity and sensitivity. This sandwich ELISA design employs a Bevacizumab specific monoclonal antibody for capture and a specific monoclonal antibody for detection.
Sensitivity
<2ng/ml
Specificity: |hIgG1, rituximab, and infliximab prepared at 250ng/mL were assayed and exhibited no crossreactivity or interference.
Recovery: |250ng/mL of Bevacizumab was spiked in 10 lots of human serum. Recovery ranges are from 91-117% with an average recovery of 106%.
Precision:t|Intra-assay CV: <10% |Inter-assay CV: <10%.
Test Principle
This assay employs the sandwich enzyme immunoassay technique. Anti- Bevacizumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Bevacizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Bevacizumab present in test samples.
Kit Components
Coated microtiter plate, 1x96 wells Calibrator diluent, 1x1.8mlCalibrator (0.25mg/mL), 1x12ulWash buffer, 10X, 1x25mlAssay buffer 1x50mlDetection reagent, 1000X, 1x17ulTMB, 1x12mlTMB stop solution, 1x12ml
Storage and Stability
Store kit components at -20°C unless specified otherwise. Unopened kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge small tubes on pulse setting prior to opening.
