Oxaloacetate(OAA)isanintermediateinthecitricacidcycleandparticipatesingluconeogenesis.OAAisformedbytheoxidationofmalate,bydeamidationofaspartateorbycondensationofCO2withpyruvateorphosphoenolpyruvate.TheOxaloacetateAssayKitprovidesasimple,directandautomation-readyprocedureformeasuringoxaloacetateconcentration.OAAisconvertedintopyruvatewhichisthenoxidizedwiththeconversionofthedyeintoacoloredandfluorescentform.Thecolorintensityoftheoxidizeddyeat570nmor|fluorescenceintensityatlex/em=530/585nmisdirectlyproportionaltotheoxaloacetateconcentrationinthesample.

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