MaxLight™550isanewYellow-GreenphotostabledyeconjugatecomparabletoAlexaFluor™546,555,DyLight™549,Cy3™,TRITCandoffersbetterlabelingefficiency,brighterimagingandincreasedimmunodetection.Absorbance(550nm);Emission(575nm);ExtinctionCoefficient150,000.
Detectsabandofthepropermolecularweight,30kD,inlysatesfromE.coliexpressingGFP,butnotinE.colithatdonotexpressorinlysatespreparedfromE.colicontainingtheGFP-expressingplasmidbutnotinduced,detectGFPinlysatesfromCOSorprimaryneuronsorfibroblastcellstransfectedwithplasmidsdirectingexpressionGFPoratau-GFPfusionprotein.Didnotdetectanysignalwhen50ugofproteinfromaratbrainlysate(negativecontrol)wasfractionatedontheSDS-PAGE.
Applications:|SuitableforuseinWesternBlot,ImmunocytochemistryandImmunoprecipitation.Otherapplicationshavenotbeentested.
RecommendedDilutions
Immunocytochemistry:Cellswerefixedusing4%formaldehydeinPBS,pH7.4for20minutesatRT.Thecellsareperme
ABIlizedwith0.2%TritonX-100and2%serum(correspondingtotheanimalinwhichthesecondaryantibodywasraised)inPBSfor20minutesatRT.CellculturesarethenincubatedwithprimaryantiserainPBScontaining2%serum(asabove),at4°C,overnight.Hasalsobeenusedsuccessfullywiththeavidin/biotin/peroxidasedetectionsystem.Cultureswerewashedfourtimes(10minuteseach)betweensteps,andreactionproductisdevelopedwitha0.05%diaminobenzidine/0.02%hydrogenperoxidesolutiononicefor8-10minutes.Optimaldilutionstobedeterminedbytheresearcher.
StorageandStability
Storeproductat4°Cinthedark.DONOTFREEZE!Stableat4°Cfor12monthsafterreceiptasanundilutedliquid.Diluterequiredamountonlypriortoimmediateuse.Furtherdilutionscanbemadeinassaybuffer.Caution:MaxLight™550conjugatesaresensitivetolight.Formaximumrecoveryofproduct,centrifugetheoriginalvialpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.
References
1.Hallam,S.J.,etal.,NatureNeuroscience5:1137-1146(2002).2.Sippy,T.,etal.,NatureNeuroscience10:1031-1038(2003).3.Jakobsson,J.,etal.,EuropeanJournalofNeuroscience19:761-765(2004).4.Zhao,S.,etal.,EuropeanJournalofNeuroscience19:1133-1140(2004).5.Yawata,S.,etal.,JournalofNeuroscience26:3626-3633(2006).
2018年8月，蚂蚁淘成为UnitedStatesBiological（简称USBio）代理商，UnitedStatesBiological也简称为USBiological。
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