PuromycinresistantGFP-HUVECswereselectedandshippedinproliferatingculturewith>90%confluence(cellsareprovidedatpassage3).ENDO-Growthmediumcontaining5%serumandgrowthsupplementisrecommendedforcellculture.Cellshaveanaveragepopulationdoublinglevelof>18whencultured.||Whenyoureceivethecells,leavetheflaskin37°CCO2incubatorfor1hour.Then,replacethetransportmediumwithfreshENDO-Growthmedium.Letthecellsgrowfor24hoursbeforesubculture.||Source:|GFPExpressingHumanUmbilicalVeinEndothelialCells,isolatedfromnormalhumanumbilicalveinsandtransfectedwithGFP-Lentiviralparticlesatpassage1.||CellNumber:|>90%confluent(>5x10e5cells)inT25flask||CellCharacterization:|CytoplasmicVWF/FactorVIII:>95%positivebyimmunofluorescence|CytoplasmicuptakeofDi-I-Ac-LDL:>95%positivebyimmunofluorescence|CytoplasmicPECAM1:>95%positivebyimmunofluorescence||GFP-HUVECsarenegativeforHIV-1,HBV,HCV,andmycoplasma.||ImportantNote:|CellsareprovidedeitherinaT-25flaskorasa1mlfrozenvial.||Store1mlvialsat-70°C.StoreT-25flasksat37°Cin5%CO2.Source:Normalhumanumbilicalveins|Purity:CellCultureGrade||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical. 2018年8月，蚂蚁淘成为UnitedStatesBiological（简称USBio）代理商，UnitedStatesBiological也简称为USBiological。 欢迎广大客户咨询订货，在线QQ：3507623898 4000-520-616 最主要的一抗产品： 144747-FITC Anti-IL-6(BCellstimulatoryfactor2)(FITC) Pab rabbit human IgG Hu FITC $592 IL6 WB 100ul 144764-FITC Anti-Interferongamma(IFN-gamma)(FITC) Pab rabbit rat IgG Rt FITC $592 IFNG E,IHC,IP,N,WB 100ul 144739-FITC Anti-IL-3(Hematopoieticgrowthfactor,Mastcellgrowthfactor)(FITC) Pab rabbit human IgG Hu FITC $592 IL3 E,IHC,IP,N,WB 100ul 144964-FITC Anti-NFKB1(KBF1,NF-kappaB,NFkappaB,NF-kB1,NFKB-p50,EBP-1,p50,p105,TRANSCRIPTIONFACTORNFKB1,DNA-bindingfactorKBF1,NFKBp50,INCLUDED,NFKBp105,INCLUDED,NuclearfactorofkappalightpolypeptidegeneenhancerinBCells1)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $628 NFKB1 WB 100ul 303092-FITC Anti-ACE(ACE1,ACET,ACE_HUMAN,ACE1,AngiotensinConvertingEnzymeSomaticIsoform,AngiotensinConvertingEnzymeTestisSpecificIsoform,AngiotensinIConvertingEnzyme,AngiotensinIConvertingEnzyme1,AngiotensinIConvertingEnzymePeptidylDipeptidaseA1,Angiotensin-convertingEnzyme,Carboxycathepsin,CD143,CD143,CD143Antigen,DCP1,DCP,DCP1,DipeptidylCarboxypeptidase1,DipeptidylCarboxypeptidaseI,KininaseII,MVCD3,PeptidaseP,PeptidylDipeptidaseA,SolubleForm,TesticularECA)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $592 IHC,WB 100ul 145142-FITC Anti-SCF(Mastcellgrowthfactor)(FITC) Pab rabbit human IgG Hu FITC $592 KITLG E,WB 100ul 145147-FITC Anti-SDHA(FlavoproteinsubunitofcomplexII)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $628 SDHA IHC,WB 100ul 145286-FITC Anti-TNC(Hxb,Hexabrachion,Tenascin-C,TN-C,TN,CYTOTACTIN,hexabrachion(tenascinC,cytotactin),hexabrachion(tenascin),MGC167029)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $628 TNC IHC,WB 100ul 303394-FITC Anti-Interleukin1Beta(IL1beta,Catabolin,H1,Hematopoietin1,IFNbetaInducingFactor,IL1,IL1beta,IL1B,IL-1beta,IL1Beta,IL1B,IL1B_HUMAN,IL1F2,Interleukin1betaPrecursor,Interleukin-1beta,LAF,OAF,OsteoclastActivatingFactor,Preinterleukin1beta,Preinterleukinbeta,ProInterleukin1beta)(FITC) Pab rabbit rat IgG Rt FITC $592 E,WB 100ul 303592-FITC Anti-SerumAmyloidP(9.5Salpha1Glycoprotein,9.5Salpha-1-glycoprotein,AmyloidPComponentSerum,APCS,PentaxinRelated,Pentraxin2,PTX2,PTX2,SAMP_HUMAN,SAP,SerumAmyloidPComponent,SerumAmyloidP-component(1-203))(FITC) Pab rabbit rat IgG Rt FITC $592 IHC,WB 100ul 144120-HRP Anti-Actin(Gamma-actin)(HRP) Pab rabbit human IgG Hu,Mo,Rt HRP $628 ACTG1 IHC,WB 100ul 144129-HRP Anti-ADAMTS2(ProcollagenIN-proteinase)(HRP) Pab rabbit human IgG Hu,Rt HRP $628 ADAMTS2 IHC,WB 100ul 144160-HRP Anti-ANGPTL3(ANG-5,ANGPT5,Angiopoietin-5)(HRP) Pab rabbit human IgG Hu,Mo,Rt HRP $628 ANGPTL3 WB 100ul 144183-HRP Anti-ApoptosisregulatorBcl-2(HRP) Pab rabbit human IgG Hu HRP $592 BCL2 E,IP,N,WB 100ul 144190-HRP Anti-AQP3(Aquaglyceroporin-3)(HRP) Pab rabbit human IgG Hu,Mo,Rt HRP $628 AQP3 IC,IHC,WB 100ul