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- 细胞因子
- Dihydropyrimidinase and Proteins
- Regulatory proteins
- 授予称号
- Peptide Substrates
- Isoform Specific Antibodies
- Deacetylase & Demethylase Proteins
- Acetyl & Methyltransferase Proteins
- siRNA Controls
- Cell Stress and Chaperone Proteins
- 激酶
- 双加氧酶与蛋白质
- Transcription Proteins
- Carbohydrate Kinases
- 表观遗传酶抑制剂
- Growth Factors
- 脂类激酶
- Phospholipase C and Proteins
- Mutant Kinases
- Oligo Substrates
- Fluorescent and Color Proteins
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商品描述
Overview:
AffinitypurifiedmouseIgGwasusedtoraisegoatantibodiesspecifictothemouseIgG.Thegoatimmuneserumissubsequentlyaffinitypurifiedusingimmunoglobulinsimmobilizedtoagarose.TheantibodiesarethenconjugatedtohorserADIshperoxidase(HRP)byreductiveaminationina2:1molarratio.TheHRPconjugatedsecondaryantibodycanrecognizetheprimaryimmunoglobulinandgiveasignalinthepresenceofachemiluminescentsubstrate.Thesignalcanbedetectedusinganelectronicimagerorautoradiographyfilm(1).
References:
1.NakanePK,PierceGB:Enzyme-labeledantibodies:preparationandapplicationforlocalizationofantigens,JHistochemCytochem1966,14:929
