- Peptide Substrates
- Binding Proteins
- Secondary Antibodies
- Regulatory proteins
- 脂类激酶
- 双加氧酶与蛋白质
- 脂质底物
- E2
- Assay Buffer and Co-factors
- Methyltransferases
- Acetyltransferases
- Transcription Proteins
- COVID-19 ELISA Kits
- Tau Proteins
- Microtubule & Actin Associated Proteins
- Carbohydrate Substrates
- COVID-19 Proteins
- Chemokines
- 标记抗体
- 授予称号
- E3
Overview:
Lysyl-endopeptidase(Lys-c)wasisolatedfromtheGram-negativesoilbacteriumAchromobacterlyticusbyMasakietal.TheproteinhydrolyzesamideandpeptideesterbondsatthecarboxylicsideoflysineandS-aminoethylcysteineresiduesmakingitanimportanttoolforenzymaticproteinsequencingandLys-Xcompoundsynthesis.Theenzymefunctionsoptimallybetween30–37oCandsuffersfromdegradationwhensubjectedtotemperaturesabove50oC.Lysyl-endopeptidaseretainscompleteactivityafterincubationin4Mureaorin0.1%SDSsolutionforupto6hoursat30oC.Conversely,theenzymeisinhibitedbyDFP,PMSF,TLCK.Lysyl-endopeptidaseisinvolvedduringtheproductionofrecombinantinsulinandinproteomicsresearch.ImmobilizedLysyl-Endopeptidaseiscovalentlycross-linkedtoanagarosematrixcircumventingtheneedforenzymeremovalaftercleavage.Theresincanbereused10-20timesafterregeneratingwhenproperstorageconditionsarefollowed.
GeneAliases:
API,ProteaseI,Lysylendopeptidase
