- Peptide Substrates
- Binding Proteins
- Secondary Antibodies
- Regulatory proteins
- 脂类激酶
- 双加氧酶与蛋白质
- 脂质底物
- E2
- Assay Buffer and Co-factors
- Methyltransferases
- Acetyltransferases
- Transcription Proteins
- COVID-19 ELISA Kits
- Tau Proteins
- Microtubule & Actin Associated Proteins
- Carbohydrate Substrates
- COVID-19 Proteins
- Chemokines
- 标记抗体
- 授予称号
- E3
Overview:
PDK1(3-phosphoinositide-dependentproteinkinase)isactivatedbythepresenceofPtdIns(3,4,5)P3orPtdIns(3,4)P2(1).PDK1thenactivatesproteinkinaseB(PKB)which,inturn,inactivatesglycogensynthasekinase-3(GSK3).PhosphorylationofotherproteinsbyPKBandGSK3islikelytomediatemanyoftheintracellularactionsofinsulin.Thus,PDK1playsakeyroleinmediatingmanyoftheactionsofthesecondmessenger(s)PtdIns(3,4,5)P3and/orPtdIns(3,4)P2.ThehumanPDK1isa556-residuemonomericenzymecomprisingofacatalyticdomainthatismostsimilartothePKA,PKBandPKCsubfamilyofproteinkinases.PDK1activityisdependentonphosphorylationatSer241.OtherknownphosphorylationtargetsitesincludeSer25,Ser393,Ser396andSer410(3).
References:
1.Cohen,P.etal:PDK1,oneofthemissinglinksininsulinsignaltransduction?FEBSLetter.1997Jun23;410(1):3-10.Review.2.Alessi,DR.etal:Characterizationofa3-phosphoinositide-dependentproteinkinasewhichphosphorylatesandactivatesproteinkinaseBalpha.CurrBiol.1997Apr1;7(4):261-9.3.CasamayorA.etal:PhosphorylationofSer-241isessentialfortheactivityof3-phosphoinositide-dependentproteinkinase-1:identificationoffivesitesofphosphorylationinvivo.BiochemJ.1999Sep1;342(Pt2):287-92.
