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- Protein Expression|Protein Expression & Purification Technologies|Protein Purification
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- 表观遗传学
- DNA Modifying Enzymes and Cloning Technologies
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- Nucleic Acid Purification|Protein Expression & Purification Technologies|RNA Reagents
- Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
- Buffers|DNA Modifying Enzymes and Cloning Technologies
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- DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
- Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
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【求助】NEB HIFI酶 双酶切
请问最近新买了NEB 的HiFi酶 分别是sac1 和Bamh1两种酶，说明书上写着是5分钟快速酶切。请问大家有没有尝试这种酶的，双酶切实际 时间和用量分别是多少呢？谢谢大家。
小刁315 2014-11-15
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Try to use CutSmart buffer for Sac I (1 ul) to cut 1-5 ug of plasmid DNA at 37 C for 2 hours in a reaction with final volume around 20-50 ul.Set up a quite same reaction for BamH I to cut your plasmid DNA.
全部回答 （共 2 条）
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