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- Protein Expression|Protein Expression & Purification Technologies|Protein Purification
- 表观遗传学|限制性内切酶
- DNA Modifying Enzymes and Cloning Technologies|Epigenetics
- Nucleic Acid Purification|RNA Reagents
- 表观遗传学
- DNA Modifying Enzymes and Cloning Technologies
- Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
- Buffers|Markers & Ladders|RNA Reagents
- Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment
- Nucleic Acid Purification|Protein Expression & Purification Technologies|RNA Reagents
- Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
- Buffers|DNA Modifying Enzymes and Cloning Technologies
- Buffers|Competent Cells
- DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
- Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
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NEB双酶切，NheI和NsiI
请问有没有谁双酶切过这两个酶切位点：NheI和NsiI，所用buffer分别是2.1和3.1，两个酶在对方的buffer里的活性只有10%，同时切的时候是不是会影响酶切效率？有没有大家常用的高效的酶切体系？要是考虑先做单酶切呢？
一只疯狂的猫 2016-03-04
全部回答 （共 3 条）
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