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找不到页面非常抱歉，我们找不到您请求的 URL。您是否在寻找特定的方法？如果是这样，您可以尝试我们的应用程序部分。使用\"产品”选项卡可以轻松访问我们的产品组合。需要额外的帮助吗？我们的\"工具与资源”选项卡中提供了多种工具、表格和视频供您选择。寻找有关 NEB 的更多信息？尝试关于 NEB 部分。如果这些都不起作用，您可以随时尝试我们的搜索引擎。Accept cookiesWe use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement.Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationLearn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus.Product Price ListDownload a PDF containing pricing for our full product list.Free ShippingSave time and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 7:30pm EST for overnight delivery.For shipping questions click here.The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptases (RTs) use an RNA template and a short primer complementary to the 3\' end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR). This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes. Alternatively, the first-strand cDNA can be made double-stranded using DNA Polymerase I and DNA Ligase. These reaction products can be used for direct cloning without amplification. In this case, RNase H activity, from either the RT or supplied exogenously, is required.Many RTs are available from commercial suppliers. Avian Myeloblastosis Virus (AMV) Reverse Transcriptase and Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase are RTs that are commonly used in molecular biology workflows. M-MuLV Reverse Transcriptase lacks 3 5 exonuclease activity. ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The enzyme is active up to 50 C, providing higher specificity, higher yield of cDNA and more full-length cDNA product, up to 12 kb in length.The use of engineered RTs improves the efficiency of full-length product formation, ensuring the copying of the 5\' end of the mRNA transcript is complete, and enabling the propagation and characterization of a faithful DNA copy of an RNA sequence. The use of the more thermostable RTs, where reactions are performed at higher temperatures, can be very helpful when dealing with RNA that contains high amounts of secondary structure.For help comparing the RTs and cDNA Synthesis reagents available, view our RT/cDNA Synthesis selection chart.This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.For more information about commercial rights, please contact NEB\'s Global Business Development team at gbd@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.If you don t see your country above, please visit ourinternational siteSession ExpiredYou have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.Sign InYour profile has been mapped to an Institution, please sign back for your profile updates to be completed.Sign InTo save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
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