Description:

 Peptide: N-Glycosidase F, alsoknown as PNGase F, is a recombinant amidasewhich cleaves between the innermost GlcNAc andasparagine residues of high mannose, hybrid,and complex oligosaccharides from N-linkedglycoproteins (1).

Product Source

Cloned from Elizabethkingia miricola(formerly Flavobacterium meningosepticum) andexpressed in E. coli (2).

Specificity

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Glycoprotein Denaturing Buffer	-20	10X
GlycoBuffer 2	-20	10X
NP-40	-20	10%

Notes:

To deglycosylate a native glycoprotein,longer incubation time as well as more enzymemay be required.Since PNGase F, Recombinant activity is inhibitedby SDS, it is essential to have NP-40 in the reactionmixture. It is not known why this non-ionicdetergent counteracts the SDS inhibition at thepresent time.PNGase F, Recombinant will not cleave N-linkedglycans containing core α1-3 Fucose.

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