Description:

Product Source

An E.coli strain that carries the cloned BtgZI gene from Bacillus thermoglucosidasius (X. Pan)

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
CutSmart® Buffer	-20	10X

Notes:

Cleavage of mammalian genomic DNA is impaired by CpG methylation.BtgZI can remain bound to DNA after cutting and alter migration rate of DNA during electrophoresis. To disrupt binding, add SDS to a final concentration of 0.5% or purify DNA before electrophoresis.Based on the stability of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.Star activity may result from a glycerol concentration of >5%

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