主页表格RNase-free Zone Sticker Pack申请表RNase-free Zone Sticker Pack申请表感谢您的申请。请等待 4-6 周的交货时间。如果您有任何问题，请随时通过 info@NEB.com 联系我们。视频 +避免 RNase 污染

了解 RNase 污染的来源以及如何防止它们干扰您的实验。

相关链接RNA 相关工作流程RNA 技术指南支持 Covid-19 研究RNA Metro 地图海报创新酶HomeFormsRNase-free Zone Sticker Pack Request FormRNase-free Zone Sticker Pack Request Form 请填写以下信息以获得免费的 RNase-free 区域贴纸包。该包装包含 2 个大贴纸和 16 个小贴纸，用于识别实验室中无 RNase 区域的特定区域和设备。每位顾客限购一份，送完即止。查看下面的条款和条件了解详情。

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视频 +避免 RNase 污染

了解 RNase 污染的来源以及如何防止它们干扰您的实验。

相关链接RNA 相关工作流程RNA 技术指南支持 Covid-19 ResearchRNA Metro Map 海报酶促创新主页试剂和分子生物学产品RNA 试剂产品RNase 对照产品 RNase ControlProduct ListingProduct Overview

NEB 提供三种不同的 RNase 抑制剂。

选择产品：RNase Control RNase ControlRNase ControlCloseRibonucleoside Vanadyl ComplexCloseRNase Inhibitor, Human PlacentaCloseRNase Inhibitor, MurineSubmit Restocking OrderIneligible item added to cart

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继续HomeResearch LabsCorrêa LabCorrêa Lab 通过质谱法进行 RNA 测序和修饰作图 通过质谱法进行 RNA 测序和修饰作图

RNA 修饰在现存生活中无处不在。已经表征了超过 150 种不同的转录后 RNA 修饰。将 RNA 测序、化学操作、质谱和生物信息学结合在一起的新方法扩展了我们对表观转录组及其如何受发育和环境变化影响的理解。然而，大多数修饰的精确位置、化学计量、串扰和功能仍然难以捉摸。开发用于识别核糖核苷修饰的整体并绘制其位置的技术是一个具有巨大发现和创新潜力的活跃研究领域。目前的策略基于使用一种或多种内切核糖核酸酶对 RNA 进行特异性切割，以生成合适的片段用于质量光谱分析。欧您的研究旨在改进无偏 RNA 测序的实验和计算方案。此外，我们渴望发现和表征新的核糖核酸酶特异性和 RNA 修饰酶。

RNA 修饰工作流程示意图质谱作图< /p>

我们正在积极寻求通过结合质谱和测序技术来映射 RNA 修饰的创新策略。我们应用比较和从头方法来映射合成和天然 RNA 序列中的修饰。我们还在推进工作流程，以实现完整重建长而复杂的 RNA 中的序列和修饰模式，包括基于 RNA 的疗法和疫苗。

主页试剂和分子生物学产品 RNA 试剂产品 RNA 标记物和梯子产品 RNA Markers & LaddersProduct ListingProduct Overview

NEB 提供多种 RNA Markers 和 Ladders，大小范围从 17 到 9,000 个碱基。

选择产品：RNA Markers & Ladders RNA Markers & LaddersRNA Markers & LaddersClosedsRNA LadderCloseLow Range ssRNA LadderClosemicroRNA Marker CloseRNA Loading Dye, (2X)ClosesRNA LadderSubmit Restocking OrderIneligible item added to cart

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继续主页试剂和分子生物学产品标记和梯子产品RNA 标记和梯子产品 RNA Markers & Ladders产品列表产品概述NEB 精选的 DNA、RNA 和蛋白质标记物和 ladders 结合了高品质和卓越的价值。 DNA、RNA 和蛋白质标记物有多种尺寸可供选择，并以方便的未染色或预染色形式提供。选择产品：RNA Markers & Ladders RNA Markers & LaddersRNA Markers & LaddersClosedsRNA LadderCloseLow Range ssRNA LadderClosemicroRNA MarkerCloseRNA Loading Dye, (2X)ClosessRNA LadderSubmit Restocking OrderIneligible item added to cart

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继续Accept cookies We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement. Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103) Even more from less, for DNA Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles. Get more of what you need, with the highest library yields Use to generate high quality librariesfrom a broad range of input amounts, from 500 pg to 1 g Prepare libraries from ALL your samples, including GC-rich DNAand FFPE DNA samples Improve yields and quality for target enrichment applications Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers. This kit is available withand withoutsample purification beads. To find out how to order this product from your current location, click the button below: International Customer Order Support The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities.The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA. Now also available with optional SPRIselect beadsfor clean-up and size-selection steps. Advantages Get more of what you need, with the highest library yields Use to generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples Improve yields and quality for target enrichmentapplications Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats To learn more about how NEBNext Ultra II addresses lower input amounts and challenging sample types, download our technical note. For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance. NEBNext Ultra II produces the highest library yields and conversion rates from a broad range of input amounts A: Libraries were prepared from Human NA19240 genomic DNA using the input amounts and numbers of PCR cycles shown. Manufacturers recommendations were followed, with the exception that size selection was omitted. B: Libraries were prepared from Human NA19240 genomic DNA using the input amounts and library prep kits shown without an amplification step, following manufacturers\' recommendations. qPCR was used to quantitate adaptor-ligated molecules, and quantitation values were then normalized to the conversion rate for Ultra II. The Ultra II Kit produces the highest rate of conversion to adaptor-ligated molecules, for a broad range of input amounts. View additional data on library yield NEBNext Ultra II provides uniform GC coverage for microbial genomic DNA over a broad range of GC composition and input amounts Libraries were made using 500 pg, 1 ng and 100 ng of the genomic DNAs shown and the Ultra II DNA Library Prep Kit and sequenced on an Illumina MiSeq . Reads were mapped using Bowtie 2.2.4 and GC coverage information was calculated using Picard\'s CollectGCBiasMetrics (v1.117). Expected normalized coverage of 1.0 is indicated by the horizontal grey line, the number of 100 bp regions at each GC% is indicated by the vertical grey bars, and the colored lines represent the normalized coverage for each library. View additional data on library quality NEBNext Ultra II provides superior yields in PCR-free workflows Libraries were generated from 100 ng of Human NA19240 genomic DNA using the library prep kits shown, following manufacturers\' recommendations, and with no amplification step. Library yields were determined by qPCR using the NEBNext Library Quant Kit for Illumina. The NEBNext Ultra II Kit produces the highest yields. NEBNext Ultra II libraries provide the highest quality sequence data in PCR-free workflows PCR-free libraries were generated from 100 ng of Human NA19240 genomic DNA using library prep kits shown, following manufacturers recommendations, and with no amplification step. Libraries were sequenced on the Illumina NextSeq 500. Reads were mapped to the GRCh37 reference using Bowtie 2.2.4.This data illustrates that NEBNext Ultra II DNA Library Prep Kit enables high quality sequence data in PCR-free workflows, even with low input amounts. % Mapped:The percentage of reads mapped to human GRCh37 reference. % Duplication:The percentage of mapped sequence that is marked as duplicate. % Chimeras:The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes. NEBNext Ultra II enables construction of high quality libraries from FFPE DNA samples Libraries were prepared from 17 30 ng of human DNA extracted from the FFPE tissue samples listed, amplified using 10 cycles of PCR and sequenced on the Illumina MiSeq. Reads were mapped to the GRCh37 reference genome using Bowtie 2.2.4. % Mapped:The percentage of reads mapped to Human GRCh37 reference. % Mapped in Pairs:The percentage of reads whose mate pair was also aligned to the reference. % Duplication:The percentage of mapped sequence that is marked as duplicate. % Chimeras:The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes. This data illustrates that the NEBNext Ultra II DNA Library Prep Kit for Illumina enables high quality sequence data, even with low input amounts of FFPE DNA. View additional data from FFPE DNA samples NEBNext Ultra II provides superior yields for target enrichment applications. For Nimblegen enrichment, libraries were prepared with 100 ng of Human HG02922 genomic DNA, using NEBNext Ultra II with the NEBNext Adaptor and index primers, or the Kapa Library Preparation Kit Illumina Platforms with the SeqCap Adapter Kit (Roche). Target enrichment was performed with NimbleGen SeqCap Human Exome v3. For SureSelectXT enrichment, libraries were prepared with 200 ng of Human HG02922 genomic DNA, using NEBNext Ultra II or Kapa Hyper with the NEBNext Adaptor and index primers, or the SureSelectXT Reagent Kit, Illumina (ILM) platforms with Herculase II Fusion DNA Polymerase. Target enrichment was performed with SureSelect Human All Exon V6. Manufacturers recommendations were followed, including use of the recommended number of PCR cycles for pre- and post-capture. NEBNext Ultra II libraries resulted in the highest post-capture yields for both enrichment workflows. View additional data on target enrichment Lot Control The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page Kit Components The following reagents are supplied with this product: 0.1X TE (1 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) DNA LoBind Tubes (Eppendorf #022431021) NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600, #E6609, #E7710, or #E7730) Magnetic rack or plate (e.g., NEBNext Magnetic Separation Rack (NEB #S1515S), Alpaqua 96S Super Magnet Plate (#A001322), or equivalent) PCR Machine For NEB #E7645 only: SPRIselect Reagent Kit (Beckman Coulter, Inc. #B23317) or AMPure XP Beads (Beckman Coulter, Inc. #A63881) Optional: 10 mM Tris-HCl, pH 8.0 or Tris/NaCl, pH 8.0 (10 mM Tris, 10 mM NaCl) (for adaptor dilution of DNA input 100 ng) The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualE7103-E7645 Gudbjartsson, D.F., et al. (2020) Early spread of SARS-Cov-2 in the Icelandic population medRxiv; DOI: https://doi.org/10.1101/2020.03.26.20044446 DNA Pipelines R D, et al. (2020) COVID-19 ARTIC v3 Illumina library construction and sequencing protocol V.3 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bgq3jvyn Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] E7645S_L_v1 This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.For more information about commercial rights, please contact NEB\'s Global Business Development team at gbd@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.New England Biolabs (NEB) is committed to practicing ethical science we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more. LicensesThis product is covered by one or more patents. This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows. For additional information or to inquire about commercial use, please contact busdev@neb.com. Ineligible item added to cart Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8. 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