Catalog #5005
PureCol® is a 3 mg/ml, Type I bovine atelocollagen solution for 2D coatings, 3D hydrogels, or as a collagen standard. PureCol® is known as the standard among collagen products for purity (≥99.9% collagen content), consistency and reproducibility, and is cited in 2000 publications.
Product DescriptionPureCol® collagen is known as the standard of all collagens for purity ( 99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
PureCol® collagen is approximately 97% Type I atelocollagen with the remainder being comprised of Type III collagen. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.
Parameter, Testing, and Method
PureCol® Type I Collagen #5005
Sterilization Method
Filtration
Extraction Method
Enzyme - atelocollagen
Form
Solution
Package Size
100 mL, 1000 mL
Storage Temperature
2-10°C
Shelf Life
Minimum of 6 months from date of receipt
Collagen Concentration - Biuret
2.9-3.2 mg/mL
Collagen Purity - Silver Staining
99.9%
pH
1.9-2.1
Kinetic Gel Test (Minutes)
40
Gel Formation Tube Test (Minutes)
40
Fibrillogenesis (Absorbance Units)
0.5
Electrophoretic Pattern - Coomassie Blue
Characteristic
Sterility - USP modified
No growth
Endotoxin - LAL
1.0 EU/mL
Osmolality (mOsmo H2O/kg)
35
Cell Attachment Assay
Pass
Source
Bovine Hide
Hydrogel Young s Modulus E (Pa)
Characteristic
Product ReferencesBecause PureCol® has been cited in over 2000 publications, we have only posted a few below:
Sorensen, Jacob R., et al. An altered response in macrophage phenotype following damage in aged human skeletal muscle: implications for skeletal muscle repair. The FASEB Journal (2019): fj-201900519R.
Sorensen, Jacob R., et al. An altered response in macrophage phenotype following damage in aged human skeletal muscle: implications for skeletal muscle repair. The FASEB Journal (2019): fj-201900519R.
Colaço, E., et al. Hierarchical Collagen-Hydroxyapatite Nanostructures Designed Through Layer-by-Layer Assembly of Crystal-Decorated Fibrils. J., Hierarchical Collagen-Hydroxyapatite Nanostructures Designed Through Layer-by-Layer Assembly of Crystal-Decorated Fibrils (May 13, 2019)(2019).
Schwerdtfeger, Luke A., et al. Human colon function ex vivo: Dependence on oxygen and sensitivity to antibiotic. PloS one14.5 (2019): e0217170.
Cardoso, Ana, et al. MiR-144 overexpression as a promising therapeutic strategy to overcome glioblastoma cell invasiveness and resistance to chemotherapy. Human molecular genetics (2019).
Steele, Hannah E., et al. Mechanotransduction of mitochondrial AMPK and its distinct role in flow-induced breast cancer cell migration. Biochemical and biophysical research communications 514.2 (2019): 524-529.
Gehwolf, Renate, et al. Global Responses of Il-1β-Primed 3D Tendon Constructs to Treatment with Pulsed Electromagnetic Fields. Cells 8.5 (2019): 399.
Alexander, Frank, Sebastian Eggert, and Dorielle Price. Label-Free Monitoring of 3D Tissue Models via Electrical Impedance Spectroscopy. (2019): 1-24.
Matysik-Woźniak, Anna, et al. Examination of Kynurenine Toxicity on Corneal and Conjunctival Epithelium: In vitro and in vivo Studies. Ophthalmic research (2019): 1-12.
Compton, Clayton, et al. Reconstitution of the Ventricular Endocardium Within Acellular Hearts. Regenerative Engineering and Translational Medicine (2019): 1-11.
Müller, A. L., et al. 4. Identification of miR-301a in Primary Human Atrial Fibroblasts and Bone Marrow-Derived Mesenchymal Progenitor Cells to Attenuate Endogenous Differentiation into Pro-Fibrotic Cells. Differentiation of Primary Human Pro-Fibrotic Mesenchymal Cells Influenced by Extracellular Matrix Environment Determined by Micro-RNA Expression (2018): 130.
Doblinger, Nina, et al. Impact of hydroxyethyl starch and modified fluid gelatin on granulocyte phenotype and function. Transfusion (2019).
Elisabeth, et al. Pro-Inflammatory Responses in Human Bronchial Epithelial Cells Induced by Spores and Hyphal Fragments of Common Damp Indoor Molds. International journal of environmental research and public health 16.6 (2019): 1085.
Dodmane, Puttappa R., et al. Biphasic changes in airway epithelial cell EGF receptor binding and phosphorylation induced by components of hogbarn dust. Experimental lung research 44.10 (2018): 443-454.
McClellan, Alyce, et al. A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta. Scientific reports 9 (2019).
Wang, Weiling, et al. Aquaporin-3 deficiency slows cyst enlargement in experimental mouse models of autosomal dominant polycystic kidney disease. The FASEB Journal(2019): fj-201801338RRR.
Teo, Jye Yng, et al. Surface tethering of stem cells with H2O2-responsive anti-oxidizing colloidal particles for protection against oxidation-induced death. Biomaterials 201 (2019): 1-15.
Gehwolf, Renate, et al. 3D-Embedded Cell Cultures to Study Tendon Biology. (2019): 1-11.
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