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Catalog #5007

VitroCol® is a 3 mg/ml, type I human atelocollagen solution for 2D coatings, 3D hydrogels, or as a collagen standard. VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells, then processed and purified to yield the naturally produced human collagen.

Product Description

VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity ( 99% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. VitroCol® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is sterile filtered and is supplied as a ready to use solution.

Parameter, Testing, and Method

VitroCol® Type I Collagen #5007

Sterilization Method

Filtration

Extraction Method

Enzyme - atelocollagen

Form

Solution

Package Size

20 mL, 100 mL

Storage Temperature

2-10°C

Shelf Life

Minimum of 6 months from date of receipt

Collagen Concentration - Biuret

2.9-3.2 mg/mL

Collagen Purity - Silver Staining

99%

pH

1.9-2.1

Kinetic Gel Test (Minutes)

40

Gel Formation Tube Test (Minutes)

40

Fibrillogenesis (Absorbance Units)

0.5

Electrophoretic Pattern - Coomassie Blue

Characteristic

Sterility - USP modified

No growth

Endotoxin - LAL

5.0 EU/mL

Osmolality (mOsmo H2O/kg)

35

Cell Attachment Assay

Pass

Source

Human Neo-Natal Fibroblasts

Hydrogel Young s Modulus E (Pa)

Characteristic

Product References

References for VitroCol®:

Shieh, Hester F., et al. Comparisons of human amniotic mesenchymal stem cell viability in FDA-approved collagen-based scaffolds: Implications for engineered diaphragmatic replacement. Journal of pediatric surgery 52.6 (2017): 1010-1013.

van der Velden, Jos LJ, et al. Transforming Growth Factor-ß Induces A Mesenchymal Profile In Human Nasal Epithelial Cells. D76. ALVEOLAR EPITHELIUM: NOVEL TOOLS AND PHENOTYPES. American Thoracic Society, 2012. A6322-A6322.

Tashima, Takumi, et al. Osteomodulin regulates diameter and alters shape of collagen fibrils. Biochemical and biophysical research communications 463.3 (2015): 292-296.

Spiller, Kara L., Suzanne A. Maher, and Anthony M. Lowman. Hydrogels for the repair of articular cartilage defects. Tissue engineering part B: reviews 17.4 (2011): 281-299.

Brilha, Sara, et al. Monocyte adhesion, migration, and extracellular matrix breakdown are regulated by integrin αVβ3 in Mycobacterium tuberculosis infection. The Journal of Immunology 199.3 (2017): 982-991.

Jonsdottir, Hulda R., and Ronald Dijkman. Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. Coronaviruses. Humana Press, New York, NY, 2015. 73-87.

Sabbione, Florencia, et al. Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells. Journal of innate immunity 9.4 (2017): 387-402.

Dos Santos Brilha, S., et al. Monocyte adhesion, migration and extracellular matrix breakdown is regulated by integrin αVβ3 in Mycobacterium tuberculosis infection.

Brilha, Sara, et al. Monocyte Adhesion, Migration, and Extracellular Matrix Breakdown Is Regulated by Integrin aVb3 in Mycobacterium tuberculosis Infection. (2017).

Colace, T., et al. Analysis of morphology of platelet aggregates formed on collagen under laminar blood flow. Annals of biomedical engineering 39.2 (2011): 922-929.

Muthard, Ryan W., and Scott L. Diamond. Blood clots are rapidly assembled hemodynamic sensors: flow arrest triggers intraluminal thrombus contraction. Arteriosclerosis, thrombosis, and vascular biology 32.12 (2012): 2938-2945.

Maloney, S. F., Lawrence F. Brass, and S. L. Diamond. P2Y12 or P2Y1inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions. Integrative Biology 2.4 (2010): 183-192.

Bauer, Rebecca N., et al. Interaction with epithelial cells modifies airway macrophage response to ozone. American journal of respiratory cell and molecular biology 52.3 (2015): 285-294.

Kawamura, Shunsuke, et al. Identification of common monocyte progenitors, pre-monocytes, and granulocyte monocyte progenitors in human umbilical cord blood. Experimental Hematology 43.9 (2015): S72.

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