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蚂蚁淘在线 / 品牌中心 / Novus / Novus/Recombinant Human IL-4 Protein/50 ug/204-IL-050

Novus/Recombinant Human IL-4 Protein/50 ug/204-IL-050

价格
￥13580.00
货号：204-IL-050
浏览量：127
品牌：Novus
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商品描述

RecombinantHumanIL-4ProteinSummary

AdditionalInformation
ANewrhIL-4isNowAvailable!Thenewproteinismammaliancellexpressed!
DetailsofFunctionality
MeasuredinacellproliferationassayusingTF‑1humanerythroleukemiccells.Kitamura,T.etal.(1989)J.CellPhysiol.140:323.TheED50forthiseffectis0.05-0.2ng/mL.ThespecificactivityofrecombinanthumanIL-4isapproximately2.9x104IU/μg,whichiscalibratedagainsthumanIL-4WHOInternationalStandard(NIBSCcode:88/656).
Source
E.coli-derivedhumanIL-4proteinHis25-Ser153,withanN-terminalMet
Accession#
P05112
N-terminalSequence
Met
Protein/PeptideType
RecombinantProteins
Gene
IL4
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
EndotoxinNote
<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
15kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
14kDa,reducingconditions
ReviewedApplications
Read2Reviewsrated5
using204-ILinthefollowingapplication:
  • CellProliferation
Publications
ReadPublicationsusing204-ILinthefollowingapplications:
  • Bioassay
    305publications
  • CellCulture
    9publications
  • ELISA(Standard)
    8publications
  • ExVivo
    1publication

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninPBSwithBSAasacarrierprotein.
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
ReconstitutionInstructions
Reconstituteat100-200μg/mLinsterilePBScontainingatleast0.1%humanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanIL-4Protein

  • Bcellgrowthfactor1
  • BCDF
  • B-cellstimulatoryfactor1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cellstimulatoryfactor1
  • interleukin4
  • interleukin-4
  • Lymphocytestimulatoryfactor1
  • MGC79402
  • pitrakinra

Background

Interleukin-4(IL-4),alsoknownasBcell-stimulatoryfactor-1,isamonomeric,approximately13kDa‑18kDaTh2cytokinethatshowspleiotropiceffectsduringimmuneresponses(1‑3).Itisaglycosylatedpolypeptidethatcontainsthreeintrachaindisulfidebridgesandadoptsabundledfouralpha-helixstructure(4).HumanIL-4issynthesizedwitha24aasignalsequence.Alternatesplicinggeneratesanisoformwitha16aainternaldeletion.MaturehumanIL-4shares55%,39%and43%aasequenceidentitywithbovine,mouse,andratIL-4,respectively.Human,mouse,andratIL-4arespecies-specificintheiractivities(5‑7).IL-4exertsitseffectsthroughtworeceptorcomplexes(8,9).ThetypeIreceptor,whichisexpressedonhematopoieticcells,isaheterodimeroftheligandbindingIL-4Ralphaandthecommongammachain(asharedsubunitofthereceptorsforIL-2,-7,-9,-15,and‑21).ThetypeIIreceptoronnonhematopoieticcellsconsistsofIL-4RalphaandIL‑13Ralpha1.ThetypeIIreceptoralsotransducesIL-13mediatedsignals.IL-4isprimarilyexpressedbyTh2-biasedCD4+Tcells,mastcells,basophils,andeosinophils(1,2).Itpromotescellproliferation,survival,andimmunoglobulinclassswitchtoIgG4andIgEinhumanBcells,acquisitionoftheTh2phenotypebynaïveCD4+Tcells,primingandchemotaxisofmastcells,eosinophils,andbasophils,andtheproliferationandactivationofepithelialcells(10‑13).IL-4playsadominantroleinthedevelopmentofallergicinflammationandasthma(12,14).

  1. Benczik,M.andS.L.Gaffen(2004)Immunol.Invest.33:109.
  2. Chomarat,P.andJ.Banchereau(1998)Int.Rev.Immunol.17:1.
  3. Yokota,T.etal.(1986)Proc.Natl.Acad.Sci.83:5894.
  4. Redfield,C.etal.(1991)Biochemistry30:11029.
  5. Ramirez,F.etal.(1988)J.Immunol.Meth.221:141.
  6. Leitenberg,D.andT.L.Feldbush(1988)Cell.Immunol.111:451.
  7. Mosman,T.R.etal.(1987)J.Immunol.138:1813.
  8. Mueller,T.D.etal.(2002)Biochim.Biophys.Acta1592:237.
  9. Nelms,K.etal.(1999)Annu.Rev.Immunol.17:701.
  10. Paludan,S.R.(1998)Scand.J.Immunol.48:459.
  11. Corthay,A.(2006)Scand.J.Immunol.64:93.
  12. Ryan,J.J.etal.(2007)Crit.Rev.Immunol.27:15.
  13. Grone,A.(2002)Vet.Immunol.Immunopathol.88:1.
  14. Rosenberg,H.F.etal.(2007)J.AllergyClin.Immunol.119:1303.

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