Details of Functionality	Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6ng/mL.The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg,which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286).
Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF proteinAla27-Arg191
Accession #	NP_001165097
N-terminal Sequence	Ala27
Structure / Form	Disulfide-linked homodimer
Protein/Peptide Type	Recombinant Proteins
Gene	VEGFA
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note	<0.01 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Theoretical MW	19.2 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE	20-22 kDa, reducing conditions39-42 kDa, non-reducing conditions
Publications	ReadPublications using 293-VE in the following applications: Array Development 1 publication Binding Analysis 1 publication Binding Assay 3 publications Bioassay 272 publications Cell Culture 9 publications Differentiation 1 publication ELISA 1 publication ELISA (Capture) 2 publications ELISA (Standard) 5 publications ELISA Capture 1 publication ELISA Development 1 publication Immunoassay Development 1 publication Immunoassay Standard 1 publication In Vivo 35 publications Organ Culture 1 publication Quantum Dot 1 publication Surface Plasmon Resonance 3 publications Surface Plasmon Resonance (SPR 1 publication Western Blot 4 publications

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