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蚂蚁淘在线 / 品牌中心 / Novus / Novus/Recombinant Human TGF-beta 1 Protein/2 ug/240-B-002

Novus/Recombinant Human TGF-beta 1 Protein/2 ug/240-B-002

价格
￥5180.00
货号：240-B-002
浏览量：127
品牌：Novus
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商品描述

RecombinantHumanTGF-beta1ProteinSummary

AdditionalInformation
ANewTGF-beta1isAvailable!ThenewproteinisHek293expressed!
DetailsofFunctionality
MeasuredbyitsABIlitytoinhibittheIL-4-dependentproliferationofHT‑2mouseT cells.Tsang, M.et al.(1995)Cytokine7:389.TheED50forthiseffectis0.04-0.2ng/mL.ThespecificactivityofRecombinantHumanTGF-beta1isapproximately2.5x104U/μg,whichiscalibratedagainsthumanTGF‑beta1Standard(NIBSCcode:89/514).
Source
ChineseHamsterOvarycellline,CHO-derivedhumanTGF-beta1proteinAla279-Ser390
Accession#
P01137
N-terminalSequence
Ala279
Structure/Form
Disulfide-linkedhomodimer
Protein/PeptideType
RecombinantProteins
Gene
TGFB1
Purity
>97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining.
EndotoxinNote
<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
12.8kDa(monomer).Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
12kDa,reducingconditions24kDa,non-reducingconditions
Publications
ReadPublicationsusing240-Binthefollowingapplications:
  • Bioassay
    570publications
  • CellCulture
    9publications
  • ELISA(Standard)
    7publications
  • ELISADevelopmet
    1publication
  • ELISAStandard
    1publication
  • InVivo
    16publications
  • Standard
    1publication
  • SurfacePlasmonResonance(SPR
    1publication
  • WesternBlot
    4publications

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninAcetonitrileandTFAwithBSAasacarrierprotein.
Purity
>97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining.
ReconstitutionInstructions
PurifiedrecombinanthumanTGF-beta1isanextremelyhydrophobicproteinthatadheresstronglytosurfaces.Toensurerecovery,reconstituteat20μg/mLinsterile4mMHClcontaining1mg/mLhumanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanTGF-beta1Protein

  • CEDLAP
  • DPD1
  • latency-associatedpeptide
  • TGFbeta1
  • TGFB
  • TGFB1
  • TGF-beta1protein
  • TGFbeta1
  • TGF-beta1
  • TGFbeta
  • TGF-beta-1
  • transforminggrowthfactorbeta-1
  • transforminggrowthfactor,beta1

Background

TGF-beta1(transforminggrowthfactorbeta1)isoneofthreecloselyrelatedmammalianmembersofthelargeTGF-betasuperfamilythatshareacharacteristiccystineknotstructure(1‑7).TGF-beta1,-2and-3arehighlypleiotropiccytokinesthatareproposedtoactascellularswitchesthatregulateprocessessuchasimmunefunction,proliferationandepithelial-mesenchymaltransition(1‑4).EachTGF-betaisoformhassomenon‑redundantfunctions;forTGF-beta1,micewithtargeteddeletionshowdefectsinhematopoiesisandendothelialdifferentiation,anddieofoverwhelminginflammation(2).HumanTGF‑beta1CDNAencodesa390aminoacid(aa)precursorthatcontainsa29aasignalpeptideanda361aaproprotein(8).Afurin‑likeconvertaseprocessestheproproteintogenerateanN‑terminal249aalatency‑associatedpeptide(LAP)andaC‑terminal112aamatureTGF‑beta1(8,9).Disulfide‑linkedhomodimersofLAPandTGF‑beta1remainnon‑covalentlyassociatedaftersecretion,formingthesmalllatentTGF‑beta1complex(8‑10).CovalentlinkageofLAPtooneofthreelatentTGF‑betabindingproteins(LTBPs)createsalargelatentcomplexthatmayinteractwiththeextracellularmatrix(9,10).TGF‑betaisactivatedfromlatencybypathwaysthatincludeactionsoftheproteaseplasmin,matrixmetalloproteases,thrombospondin1andasubsetofintegrins(10).MaturehumanTGF‑beta1shares100%aaidentitywithpig,dogandcowTGF‑beta1,and99%aaidentitywithmouse,ratandhorseTGF‑beta1.Itdemonstratescross‑speciesactivity(1).TGF‑beta1signalingbeginswithhigh‑affinitybindingtoatypeIIser/thrkinasereceptortermedTGF‑betaRII.Thisreceptorthenphosphorylatesandactivatesasecondser/thrkinasereceptor,TGF‑betaRI(alsocalledactivinreceptor‑likekinase(ALK)‑5),oralternatively,ALK‑1.ThiscomplexphosphorylatesandactivatesSmadproteinsthatregulatetranscription(3,11,12).Contributionsoftheaccessoryreceptorsbetaglycan(alsoknownasTGF‑betaRIII)andendoglin,oruseofSmad‑independentsignalingpathways,allowfordisparateactionsobservedinresponsetoTGF‑betaindifferentcontexts(11).
  1. Derynck,R.andK.Miyazono(2008)ColdSpringHarborLaboratoryPress,29.
  2. Dunker,N.andK.Krieglstein(2000)Eur.J.Biochem.267:6982.
  3. Wahl,S.M.(2006)Immunol.Rev.213:213.
  4. Chang,H.etal.(2002)Endocr.Rev.23:787.
  5. Lin,J.S.etal.(2006)Reproduction132:179.
  6. Hinck,A.P.etal.(1996)Biochemistry35:8517.
  7. Mittl,P.R.E.etal.(1996)ProteinSci.5:1261.
  8. Derynck,R.etal.(1985)Nature316:701.
  9. Miyazono,K.etal.(1988)J.Biol.Chem.263:6407.
  10. Oklu,R.andR.Hesketh(2000)Biochem.J.352:601.
  11. deCaestecker,M.etal.(2004)CytokineGrowthFactorRev.15:1.
  12. Zuniga,J.E.etal.(2005)J.Mol.Biol.354:1052.

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