RunBlueFeatures&BenefitsNoveloptimisedgel-buffersystem-HighresolutionandsharpbandsExtraresolvinglength-IncreasedseparationUniquehomogeneouspolymerization-IncreasedconsistencyandnoresidualfreeacrylamideExtraWells-Runupto12or17samplessimultaneouslyDeepandwidewells-Loadupto35µl(12well)or20ul(17well)Standardformat-Compatiblewith10x10cmproteingeltankssuchasNuPAGE®XCellSureLockMini-CellProtrudingteeth-NowellcontaminationincaseofoverloADIngResilientgelmatrix-Tear-proofgelandrisk-freehandlingComb-free-NopotentiallybrokenordamagedteethStrip-free-NopotentiallyruinedWesternBlotsClippedcassette-FastandgentleopeningNeutralbufferandhighchemicalstABIlity-shelflifeof2yearsat4ºCand6monthsatRTRunBluePerformanceRunBlueSDSProteinGelstakeadvantageofExpedeon’snewmethodsandinnovationstothegelmakingprocesstoincreaseresolutionandreproducibilityofseparatedproteinbands.RunBlueSDSProteinGelsarebasedonanovelgelbuffersystemwhichprovidesaseparationprofilesimilartoNupage®Bis-Trisgels,butwithenhancedseparationofhighermolecularweightproteinsandbetteroverallresolution.RunBlueProteinGel’sproprietarypolymerizationprocessresultsinmoreuniformgelsbetweenbatches,withdecreasedvariabilityandimprovedrepeatabilityofresults.UsabilityEnhancementsInadditiontotheirhighresolutionseparationofproteinallRunBlueProteinGelshaveanumberofenhancementstoimproveandstreamlineusability.RunBlueProteinGelsareupto10timesstrongerthanconventionalhand-castgelsandcanstanduptorepeatedhandling.Thispracticallyeliminatestheriskoftornandunusablegelsencounteredduringnormalgelmanipulation.Morelanesthanstandardgelsallowsmoreproteinstoberunandwide,deepwellsallowhighervolumestobeloaded.Cassetteshavenocombortapetoremoveandnoexcessgeltotrim,allofwhichimprovesthespeedandefficiencyoftheloadingandrunningprocess.Wellsareoutlinedandnumberedforquickersampleloadingandthecassetteislabeledwithgeltype,percentageandauniquelotnumberfortraceability.Wellteethprotrudeabovethecassettetopreventsamplecontaminationbetweenwellsincaseofoverloading.FlexibleUsageTheRunBlueSDSProteinGel10x10cmcassettesaredesignedtofitanumberofgeltankssuchasInvitrogen®XCellSureLock™equipment,Hoeffer,andOwltanks.RunBlueSDSProteinGelsarehighlyoptimisedforaTEO-Tricinebuffersystemandmustberunwiththisbuffersystemtoachievegoodresults.TEO-Tricinebufferisavailableasapremixed20Xsolution,orahomemadeversioncanbemixedusingtheavailablerecipe.ThesamplesmustbepreparedwithaTEO-Clbasedsamplebufferwhichisavailableasapremade4Xsolution,orcanbehomemadewiththefreelyavailablerecipe.RunBlueProteinGelshaveanextendedshelflifeof2yearsat4ºCwhichisidealforlargestocksorinfrequentuse.Theyalsohavea6monthshelflifeatroomtemperaturewhichisconvenientwhenfridgespaceislimited.ImprovedSafetyPolyacrylamidegelsarebasedonthepolymerizationofliquidacrylamideandbis-acrylamide.Thepolymerizationisusuallyinducedbytheadditionofasourceoffreeradicalsandastabilizer(usuallyAPS&TEMED)whichinducescrosslinkformationbetweenacrylamideandbis-acrylamidemonomers.Thislocalizedchemicalinductionresultsinheterogeneouspolymerizationacrossthegelduetoacrylamideconcentrationgradientsbeinggenerated,andcanleadtothepresenceofresidualfreeacrylamideattheendofthepolymerization.Expedeonhasdevelopedauniquephysicalinductiontechnologywhichensurehomogenouspolymerizationacrossthewholegelandnoresidualfreeacrylamideaftertheendofthepolymerization.Numerousstudieshaveshowntheacrylamidemonomerisaneurotoxinandpossiblecarcinogen*.Acrylamidecanbeinhaledasadrypowder,evaporatefromliquidsolutionandsublimatefromsolidgelsifnotfullypolymerized.RunBluegelsarepolymerizedinauniquehomogenousmannerthatresultsinaconsistentgelwithminimalornoresidueaftercasting.