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Novus/INSTA-Blot Mouse Tissues/NBP2-30111

价格
¥7980.00
货号:NBP2-30111
浏览量:127
品牌:Novus
服务
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商品描述
Description
Ready-to-use PVDF membrane with 11 mouse tissue lysates (20 ug total protein per lane).
Background
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Mouse Tissues NBP2-30111 is a simple and fast solution for screening proteins from various mouse tissues. We"ve streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,...immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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PreparationMethod
INSTA-Blot™ Mouse Tissues NBP2-30111contains denatured proteins from mouse lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blottedfor 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried andpackaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol.
Application Notes
Tissues are from Balb/c mice. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail(PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20%glycerol, 0.4% B-mercaptoethanol).
Publications
ReadPublications using NBP2-30111.
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