Details of Functionality	Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is typically2.5-15 ng/mL.
Source	Mouse myeloma cell line, NS0-derived human BMP-4 proteinSer293-Arg408
Accession #	Q53XC5
N-terminal Sequence	Ser293
Structure / Form	Disulfide-linked homodimer
Protein/Peptide Type	Recombinant Proteins
Gene	BMP4
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note	<0.01 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Theoretical MW	13 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE	22-25 kDa, reducing conditions37-41 kDa, non-reducing conditions
Publications	ReadPublications using 314-BP in the following applications: Binding Assay 3 publications Bioassay 323 publications Cell Culture 14 publications Differentiation 2 publications In Ovo 1 publication In Vivo 8 publications Surface Plasmon Resonance 3 publications Tissue Culture 1 publication Western Blot 1 publication

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