Summary:

Immunogen	Full-lengthrecombinanthumancaspase-3proteinwasusedasimmunogen.TheantibodyrecognizesanepitopeinthelargedomainsubunitofCaspase-3.AssuchitwillrecognizeproCaspase-3andthelargesubunitcleavagefragment.
Specificity	TheantibodydetectsbothproCaspase-3(~32kDa)andthelargesubunitoftheactive/cleavedform(~14-21kDa)ofCaspase-3.ThelargesubunitofthecleavedformmayappearasoneortwoorevenasastackofbandsdependingonthepresenceorabsenceoftheCaspase-3pro-domain.ItishighlyrecommendedthatamaximumsensitivityECLsubstrate(Femtosensitive)beusedforefficientdetectionofthisantibodyinWesternblotapplications.
Isotype	IgG1Kappa
Clonality	Monoclonal
Host	Mouse
Gene	CASP3
Purity	ProteinGpurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot1-5ug/ml SimpleWestern1:50 ElectronMicroscopy FlowCytometry Immunocytochemistry/Immunofluorescence Immunohistochemistry1:10-1:500 Immunohistochemistry-Frozen1:10-1:500 Immunohistochemistry-Paraffin1:10-1:500 CyTOF-ready
ApplicationNotes	UsefulinImmunohistochemistry-FrozenSeeZhangetal.,andImmunohistochemistry-ParaffinSeeLeeetal.UseinImmunocytochemistry/Immunofluorescencewasreportedinthescientificliterature(PMID:23840553).UseinFLOWcytometryreportedinscientificliterature(PMID27429862).Useinelectronmicroscopyreportedinscientificliterature(PMID27450722). InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue.
PositiveControl	Caspase-3Lysate(NBL1-08710)
ReviewedApplications	Read1Reviewrated4 using NB100-56708inthefollowingapplications: Immunohistochemistry-Frozen
Publications	ReadPublicationsusing NB100-56708inthefollowingapplications: EM 1publication FLOW 3publications HEStain 1publication IB 1publication IHC 1publication IHC-Fr 2publications IHC-P 2publications WB 83publications

Packaging,Storage&Formulations:

Notes:

Caspasesareafamilyofcysteineproteasesthatarekeymediatorsofprogrammedcelldeathorapoptosis.Theprecursorformofallcaspasesiscomposedofaprodomain,andlargeandsmallcatalyticsubunits.Theactiveformsofcaspasesaregeneratedbyseveralstimuliincludingligand-receptorinteractions,growthfactordeprivationandinhibitorsofcellularfunctions.Allknowncaspasesrequirecleavageadjacenttoaspartatestoliberateonelargeandonesmallsubunit,whichassociateintoa2b2tetramertoformtheactiveenzyme.GeneforCaspase-3alsoknownasYama,CPP32,andapopaincodesfora32-kDaprotein.Caspase-3cleavesthedeathsubstratepoly(ADP-ribose)polymerase(PARP)toaspecific85kDaformobservedduringapoptosisandisinhibitablebytheCrmAprotein.OtherCaspase-3substratesincludeDNA-PK,actin,GAS2,andprocaspase-6,etc.Caspase-3isactivatedbycleavageeventsatAsp-28/Ser-29(betweenN-terminalpro-domain)andAsp-175/Ser-176(betweenlargeandsmallsubunits)togeneratealargesubunitof17-kDaandasmallsubunitof12-kDa.

AlternateNamesfor53BP1Antibody:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

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