Summary:

Description	Whendetectingphospho-IRE1Alpha(Ser-724)usingNB100-2323,itisrecommendedtonormalizeitsbandintensity/immunoreactivitywithtotal-IRE1alpha.NB100-2324andNB110-59971canbeusedforthedetectionofendogenoustotalIRE1alpha.
Immunogen	Asyntheticpeptidesurroundingthephosphorylatedserine724ofthehumanIRE1alphaprotein.[Swiss-Prot#O75460]
Modification	pSer724
Localization	Endoplasmicreticulum
Specificity	NB100-2323detectsIRE-1alphawhenphosphorylatedatSer724residue.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	ERN1
Purity	Immunogenaffinitypurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot1:1000 ELISA1:100-1:2000 Immunocytochemistry/Immunofluorescence1:10-1:500 Immunohistochemistry1:10-1:500 Immunohistochemistry-Frozen1:10-1:500 Immunohistochemistry-Paraffin1:10-1:500 Immunoprecipitation1:10-1:500
ApplicationNotes	ThisIRE1pS724antibodyisusefulforWesternblot,ELISA,andImmunohistochemistryparaffinembeddedsections(PMID:19264902).UseinIHC-Frozenreportedinscientificliterature(PMID24823368).UseinICCreportedinscientificliterature(PMID26762342).
PositiveControl	IRE1alphaAntibody(NB100-2324) PancreasLysate(NB820-59443) EpididymisLysate(NB820-59213) IRE1alphaAntibodyPack(NBP2-50067)
ControlPeptide	IRE1alphaPeptide(NB100-2323PEP)
ReviewedApplications	Read5Reviewsrated4.4 using NB100-2323inthefollowingapplications: WesternBlot
Publications	ReadPublicationsusing NB100-2323inthefollowingapplications: ELISA 1publication ICC/IF 5publications IHC 4publications IHC-Fr 1publication IHC-P 6publications IP 2publications WB 116publications

ReactivityNotes:

Mammalreactivityreportedinscientificliterature(PMID:27180817).

Packaging,Storage&Formulations:

Storage	Aliquotandstoreat-20Cor-80C.Avoidfreeze-thawcycles.
Buffer	PBS
Preservative	0.025%SodiumAzide
Concentration	1.0mg/ml
Purity	Immunogenaffinitypurified

Notes:

TakealookatIRE1alphaAntibodySamplerPack[NBP2-50067]ifyouwanttotry25ugaliquotsofphospho-IRE1alpha(Ser724)Antibody[NB100-2323SS]andtotalIRE1alphaAntibody[NB100-2324SS]beforepurchasing100ugfullvials.

AlternateNamesfor53BP1Antibody:

• EC2.7.11
• endoplasmicreticulumtonucleussignaling1
• Endoplasmicreticulum-to-nucleussignaling1
• ERtonucleussignalling1
• FLJ30999
• hIRE1p
• inositol-requiring1
• Inositol-requiringprotein1
• IRE1a
• ire1alphapSer724
• ire1alphaphosphorylation
• IRE1a
• ire1-alpha
• IRE1MGC163279
• IRE1P
• MGC163277
• phosphoire1alpha
• phospho-ire1alpha
• phospho-ire1a
• p-ire1alpha
• proteinkinase/endoribonuclease
• serine/threonine-proteinkinase/endoribonucleaseIRE1

Background:

Unfoldedproteinresponse(UPR)signaling,amechanismusedbyeukaryoticcellstocopeERstress.ItisinitiatedbythreeER-localizedproteinsensors:PERK(PKR-likeERkinase),ATF(activatingtranscriptionfactor6),andIRE1alpha(inositol-requiringenzyme1alpha).UPR-responsivegenes"stranscriptionalactivationisregulatedbyATF6andIRE1-XBP1pathways,andUPRservesthreeimportantfunctions:inhibitionofproteintranslationtorestorenormalcellfunctions;activationofsignalingtoincreaseproductionofmolecularchaperonesinvolvedinproteinfolding;andactivationofsignalingthatleadstotargetingofmisfoldedproteinsinERforubiquitinationandsubsequentdegradation;orwhenER-stressisnotrelieved,UPRleadstoapoptosis.Localizedasasingle-passtypeImembraneproteininERmembrane,IRE1alphaisubiquitouslyexpressedwithhighlevelsobservedinpancreatictissue.ItundergoesautophosphorylationandgetsADP-ribosylatedbyPARP16uponERstress,whichincreasesitskinaseaswellasendonucleaseactivities.IRE1alphasensesunfoldedproteinsintheERlumenviaitsN-terminaldomainwhichleadstoenzymeauto-activationandthereafter,theactiveendoribonucleasedomainsplicesXBP1mRNAtogenerateanewC-terminus,convertingitintoapotentUPRtranscriptionalactivator.IRE1alphainactivationinmicehasbeenshowntoresultinwidespreaddevelopmentaldefects,leADIngtointra-gestationalembryoniclethalityandERstressmediatedcelldeathisresponsIBLeforseveralpathologies.

Limitations:

• EC2.7.11
• endoplasmicreticulumtonucleussignaling1
• Endoplasmicreticulum-to-nucleussignaling1
• ERtonucleussignalling1
• FLJ30999
• hIRE1p
• inositol-requiring1
• Inositol-requiringprotein1
• IRE1a
• ire1alphapSer724
• ire1alphaphosphorylation
• IRE1a
• ire1-alpha
• IRE1MGC163279
• IRE1P
• MGC163277
• phosphoire1alpha
• phospho-ire1alpha
• phospho-ire1a
• p-ire1alpha
• proteinkinase/endoribonuclease
• serine/threonine-proteinkinase/endoribonucleaseIRE1

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