Summary:

Immunogen	HumanHIF-2alpha,correspondingtoaminoacids535-631.[UniProt#Q99814]
Specificity	ThisisspecificforHIF-2alphaanddoesnotcross-reactwithHIF-1alpha.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	EPAS1
Purity	ProteinGpurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot1-2ug/ml SimpleWestern1:100 ChromatinImmunoprecipitation ELISA1:100-1:2000 FlowCytometry1:400 Immunocytochemistry/Immunofluorescence Immunohistochemistry1:150-1:300 Immunohistochemistry-Paraffin1:150-1:300 Immunoprecipitation GelSupershiftAssay
ApplicationNotes	ThisHIF-2alpha(ep190b)antibodyisusefulforELISA,FlowCytometry,Immunohistochemistryonparaffin-embeddedsectionsandWesternBlot.InWB,itrecognizesabandatapprox.118kDarepresentingHIF-2alpha.UseinGelsuppershiftassayreportedinscientificliterature(PMID17404621) InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue. Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
TheoreticalMW	118kDa. Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
PositiveControl	KidneyLysate(NB820-59231) HIF-2alpha/EPAS1Lysate(NBL1-10286)
ReviewedApplications	Read7Reviewsrated4.3 using NB100-132inthefollowingapplications: Immunofluorescence WesternBlot
Publications	ReadPublicationsusing NB100-132inthefollowingapplications: ChIP 8publications ELISA 1publication EMSA 1publication ICC/IF 7publications IHC 13publications IHC-Fr 1publication IHC-P 14publications IP 3publications WB 71publications

ReactivityNotes:

ABIlitytouseinmouseismixedwithsomepositiveandsomenegativeresults.

Packaging,Storage&Formulations:

Storage	Aliquotandstoreat-20Cor-80C.Avoidfreeze-thawcycles.
Buffer	PBSwith1%BSA
Preservative	0.05%SodiumAzide
Concentration	1.0mg/ml
Purity	ProteinGpurified

Notes:

• Basic-helix-loop-helix-PASproteinMOP2
• BHLHE73
• bHLHe73ECYT4
• ClassEbasichelix-loop-helixprotein73
• endothelialPASdomainprotein1
• endothelialPASdomain-containingprotein1
• ep190b
• EPAS1
• EPAS-1
• HIF2A
• HIF-1alpha-likefactor
• HIF2alpha
• HIF-2alpha
• hif2aangiogenesis
• HIF2A
• HIF2AHIF-2-alpha
• HLF
• HLFHIF-1-alpha-likefactor
• hypoxia-inducIBLefactor2alpha
• Hypoxia-induciblefactor2-alpha
• MemberofPASprotein2
• MOP2
• MOP2HIF2-alpha
• PASD2PASdomain-containingprotein2

AlternateNamesfor53BP1Antibody:

Hypoxia-induciblefactors(HIFs)generatesheterodimerscomposedofalpha-subunit(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunit(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplayacriticalroleinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsandbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressionforinvolvinginthedevelopmentofnewbloodvesselsaswellasthetubularsystemoflung.HIF-2alphaplayaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinasewhichisamajorplayerinangiogenesis.HIF-2alphaactivationrequirerecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300etc.anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alpha/HIF-1alphaexpressionincreasesincertainmalignancies,wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities,whereas,HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

Hypoxia-induciblefactors(HIFs)generatesheterodimerscomposedofalpha-subunit(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunit(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplayacriticalroleinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsandbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressionforinvolvinginthedevelopmentofnewbloodvesselsaswellasthetubularsystemoflung.HIF-2alphaplayaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinasewhichisamajorplayerinangiogenesis.HIF-2alphaactivationrequirerecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300etc.anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alpha/HIF-1alphaexpressionincreasesincertainmalignancies,wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities,whereas,HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly. Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 【详细说明】  Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 上海普迈生物科技有限公司签约代理      欢迎下载下面的资料以了解此产品的相关信息！预询产品订购信息  上海普迈生物致力于提供zui高质量的产品和服务组织形态学研究与竞争力的产品 产品和服务,有相关产品的问题请直接 期货每周下午5点前发定单，正常到上海时间为3-4周 现市区2天内送到，外地以快递为准 敬请关注！ ：   :www.pumaibio.com :pumaibio :（直接客户） （经销商） 促销etonbio、polysciences、Mattek、trevigen、Chromogenix、cyguns、IrvineScientific、Innovative Cell Technologies、enzo、novus、immunochemistry、eylabs、Polyplus、NIBSC、ALZET、hitobiotec、sutter instrument、mirus、StressMarq、Avanti、jena bioscience、Neuro Probe、tedpella、avanti、Rockland、CAYMAN、transgenomic、BioLegend、ambion、omicronbio、 Assaybiotech、chondrex、lc?laboratories、cosmobio、megazyme、NeoMarkers、BTI、eylabs、berryassoc、cellagentech、TOCRIS、Relia Tech、SouthernBiotech、Biosearch technology、Lumiprobe、StemCells?Inc、biovendor、NORGEN、alomone、jena?bioscience、Gracebio、Eurogentec、Gelcompany?、invitrogen、gene?bridges、MRC-Holland、laysanbio