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蚂蚁淘在线 / 品牌中心 / Novus / Novus/Recombinant Human GM-CSF (CHO-expressed) Protein/10 ug/7954-GM-010

Novus/Recombinant Human GM-CSF (CHO-expressed) Protein/10 ug/7954-GM-010

价格
￥4380.00
货号：7954-GM-010
浏览量：127
品牌：Novus
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商品描述

RecombinantHumanGM-CSF(CHO-expressed)ProteinSummary

DetailsofFunctionality
MeasuredinacellproliferationassayusingTF‑1humanerythroleukemiccells.Kitamura,T.etal.(1989)J.CellPhysiol.140:323.TheED50forthiseffectis6-30pg/mL.
Source
ChineseHamsterOvarycellline,CHO-derivedhumanGM-CSFproteinAla18-Glu144
Accession#
P04141
N-terminalSequence
Ala18
Protein/PeptideType
RecombinantProteins
Gene
CSF2
Purity
>95%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain
EndotoxinNote
<0.01 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
14.5kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
20-35kDa,reducingconditions
Publications
ReadPublicationusing7954-GMinthefollowingapplications:
  • Bioassay
    1publication

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninPBSwithBSAasacarrierprotein.
Purity
>95%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain
ReconstitutionInstructions
Reconstituteat100μg/mLinPBScontainingatleast0.1%humanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanGM-CSF(CHO-expressed)Protein

  • colonystimulatingfactor2(granulocyte-macrophage)
  • Colony-stimulatingfactor
  • CSF
  • CSF2
  • CSF-2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophagecolony-stimulatingfactor
  • granulocyte-macrophagecolonystimulatingfactor
  • MGC131935
  • MGC138897
  • Molgramostim
  • molgramostin
  • Sargramostim

Background

GM‑CSFwasinitiallycharacterizedasafactorthatcansupporttheinvitrocolonyformationofgranulocyte‑macrophageProgenitors.Itisalsoagrowthfactorforerythroid,megakaryocyte,andeosinophilprogenitors.GM‑CSFisproducedbyanumberofdifferentcelltypes(includingTcells,Bcells,macrophages,mastcells,endothelialcells,fibroblasts,andADIpocytes)inresponsetocytokineorinflammatorystimuli.Onmaturehematopoieticcells,GM‑CSFisasurvivalfactorforandactivatestheeffectorfunctionsofgranulocytes,monocytes/macrophages,andeosinophils(1,2).GM‑CSFpromotesaTh1biasedimmuneresponse,angiogenesis,allergicinflammation,andthedevelopmentofautoimmunity(3‑5).Itshowsclinicaleffectivenessinamelioratingchemotherapy‑inducedneutropenia,andGM‑CSFtransfectedtumorcellsareutilizedascancervaccines(6,7).The22kDaglycosylatedGM‑CSF,similartoIL‑3andIL‑5,isacytokinewithacoreoffourbundledalpha‑helices(8‑12).MaturehumanGM‑CSFshares63%‑70%aminoacidsequenceidentitywithcanine,feline,porcine,andratGM‑CSFand54%withmouseGM‑CSF.GM‑CSFexertsitsBIOLOGicaleffectsthroughaheterodimericreceptorcomplexcomposedofGM‑CSFRalpha/CD116andthesignaltransducingcommonbetachain(CD131)whichisalsoacomponentofthehigh‑affinityreceptorsforIL‑3andIL‑5(13,14).Inaddition,GM‑CSFbindsanaturallyoccurringsolubleformofGM‑CSFRalpha(15).HumanGM‑CSFisactiveoncanineandfelinecellsbutnotonmurinecells(16‑18).
  1. Martinez-Moczygemba,M.andD.P.Huston(2003)J.AllergyClin.Immunol.112:653.
  2. Barreda,D.R.etal.(2004)Dev.Comp.Immunol.28:509.
  3. Eksioglu,E.A.etal.(2007)Exp.Hematol.35:1163.
  4. Cao,Y.(2007)J.Clin.Invest.117:2362.
  5. Fleetwood,A.J.etal.(2005)Crit.Rev.Immunol.25:405.
  6. Heuser,M.etal.(2007)Semin.Hematol.44:148.
  7. Hege,K.M.etal.(2006)Int.Rev.Immunol.25:321.
  8. Kaushansky,K.etal.(1992)Biochemistry31:1881.
  9. Diederichs,K.etal.(1991)Science254:1779.
  10. Cantrell,M.A.etal.(1985)Proc.Natl.Acad.Sci.82:6250.
  11. Lee,F.etal.(1985)Proc.Natl.Acad.Sci.82:4360.
  12. Wong,G.G.etal.(1985)Science228:810.
  13. Onetto-Pothier,N.etal.(1990)Blood75:59.
  14. Hayashida,K.etal.(1990)Proc.Natl.Acad.Sci.87:9655.
  15. Pelley,J.L.etal.(2007)Exp.Hematol.35:1483.
  16. Hogge,G.S.etal.(1990)CancerGeneTher.6:26.
  17. Sprague,W.S.etal.(2005)J.Comp.Pathol.133:136.
  18. Shanafelt,A.B.etal.(1991)J.Biol.Chem.266:13804.

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