分析测试百科网 WIKI资讯 FluorescentNucleosideTriphosphatesforSingleMoleculeEnzymology3 2 Materials2.1 Labeling1. 2′,3′-O-(2-Aminoethyl-carbamoyl)-adenosine-5′-triphosphate (edaATP) (Jena Biosciences) (see Note 1).2. 3′-Amino-3′-deoxyATP triethylammonium salt (aminoATP) (15).3. Cy3 N-hydroxysuccinimide ester (NHS-ester) (GE Healthcare).4. 7-Diethylaminocoumarin-3-carboxylic acid (Invitrogen).5. 20 mM Sodium bicarbonate, pH 8.4.6. Dimethylfo......阅读全文 Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology-1

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