Jackson公司715-167-003/Cy™3AffiniPureFabFragmentDonke现货价格低

1.0mg

Target: MouseHost: DonkeyAntibodyFormat: FabFragmentSpecificity: IgG(H+L)Conjugate: CyanineCy™3ProductCategory: FabFragmentAffinity‑PurifiedAntibodiesClonality: Polyclonal

FabfragmentantibodiesaregeneratedbypapaindigestionofwholeIgGantibodiestoremovetheentireFcportion,includingthehingeregion.Theseantibodiesaremonovalent,containingonlyasingleantigenbindingsite.ThemolecularweightofFabfragmentsisabout50kDa.

Basedonantigen-bindingassayand/orELISA,theantibodyreactswithwholemoleculemouseIgG.Italsoreactswiththelightchainsofothermouseimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState: Freeze-driedsolid Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing. Expirationdate: oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity: Theantibodywaspurifiedfromantiserabyacombinationofpapaindigestionandimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.FcfragmentsandwholeIgGmoleculeshavebeenremoved. Buffer: 0.01MSodiumPhosphate,0.25MNaCl,pH7.6 Preservative: 0.05%SodiumAzide SuggestedWorkingConcentrationorDilutionRange:

1:100-1:800formostapplicationsDilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeABIlity,etc.Theactualdilutionusedmustbedeterminedempirically.

Cy™3AffiniPureFabFragmentDonkeyAnti-MouseIgG(H+L)

ConjugateOptions

Target: MouseHost: DonkeyAntibodyFormat: FabFragmentSpecificity: IgG(H+L)Conjugate: CyanineCy™3ProductCategory: FabFragmentAffinity‑PurifiedAntibodiesClonality: Polyclonal

Code: 715-167-003

Unit:1.0mg

Price:$145.00

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CyanineCy™3

Amax: 550 Emax: 570nm

Cy3isbrighter,morephotostable,andgiveslessbackgroundthanotherorange-redfluorescingdyeconjugates.Cy3conjugatescanbeexcitedmaximallyat550nm,withpeakemissionat570nm.Forfluorescencemicroscopy,Cy3canbevisualizedwithtraditionaltetramethylrhodamine(TRITC)filtersets,sincetheexcitationandemissionspectraarenearlyidenticaltothoseofTRITC.WerecommendCy3asabrighteralternativetoTRITC.Cy3canbeexcitedtoabout50%ofmaximumwithanargonlaser(514nmor528nmlines),ortoabout75%ofmaximumwithahelium/neonlaser(543nmline)ormercurylamp(546nmline).Cy3hasbeenusedwithfluoresceinfordoublelabeling;however,theuseofanarrowband-passemissionfilterforfluoresceinisrecommendedtominimizeCy3fluorescenceintheFITCfilterset.Cy3canalsobepairedwithAlexaFluor®647formultiplelabelingwhenusingaconfocalmicroscope.However,abetterchoiceformultiplelabelingisRhodamineRed-Xbecauseitsfluorescenceismidwaybetweenagreenfluorescingdye(likeAlexaFluor®488)andafar-red-fluorescingdyelikeAlexaFluor®647.

ThisproductisforINVITRORESEARCHUSEONLY.Itisnotamedicaldeviceanditisnotintendedfordiagnosticortherapeuticpurposes.

CyisatrademarkofGEHealthcareBio-SciencesLtd.jacksonimmunoResearchislicensedbyGEHealthcareBio-SciencesLtd.tomanufactureandsellconjugatesofCy2,Cy3,andCy5reactivedyesunderUSPatentNumber5,268,486,andotherpatentspending.

美国JacksonImmunoResearchLaboratories,Inc.专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可，销售范围遍布全球，领域包括植物，动物和生物医学研究。公司成立于1982年，公司创业者具有深厚的专业背景：细胞生物学，蛋白化学，微生物学，免疫学。公司自创立初，就将为其他研究人员提供最新最全的二抗作为公司的持续发展目标。

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