Jackson/R-Phycoerythrin AffiniPure Fab Fragment Goat Anti-Mouse IgG2a, Fcγ fragment specific/0.5 ml/115-117-186

FabfragmentantibodiesaregeneratedbypapaindigestionofwholeIgGantibodiestoremovetheentireFcportion,includingthehingeregion.Theseantibodiesaremonovalent,containingonlyasingleantigenbindingsite.ThemolecularweightofFabfragmentsisabout50kDa.

Basedonantigen-bindingassayand/orELISA,theantibodyreactswiththeFcportionofmouseIgG2abutnotwiththeFabportionofmouseimmunoglobulins.NoantibodywasdetectedagainstmouseIgMoragainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithothermouseIgGsubclassesorwithimmunoglobulinsfromotherspecies.PhysicalState:Freeze-driedsolidStorage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Storeat2-8°C-DONOTFREEZE.Prepareworkingdilutionfresheachday.Expirationdate:sixmonthsfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.Purity:Theantibodywaspurifiedfromantiserabyacombinationofpapaindigestionandimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.FcfragmentsandwholeIgGmoleculeshavebeenremoved.Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6StABIlizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)Preservative:0.05%SodiumAzideSuggestedWorkingConcentrationorDilutionRange:

1:50-1:200forsequentiallabelingapplications.

Tocomplexwithprimaryantibodyinsolution,use1:1weightratioofR-PE:primaryantibody(approximate3:1molarratioofFab:primaryantibody).Vortexandincubatefor30minutesatroomtemperaturepriortouse.Titratecomplextooptimaldilutionforassay.

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

R-PhycoerythrinAmax:488Emax:580nm

Phycoerythrin(R-PE)isamongseveralkindsoflight-harvestingphycobiliproteinsfoundinred,blue-green,andcryptomonadalgae.WeofferR-PE,theformfoundinredmacrophyticalgae(seaweed).Afterphycobiliproteinsareconjugatedtosecondaryantibodies,thereislittlefluorescencequenching,whichresultsinconjugatesofhighspecificfluorescencecomparedwithconventionalfluorophore-antibodyconjugates.R-PEcanbeexcitedbylightoverawiderangeofthevisIBLespectrumishighlywatersoluble,hasarelativelylowisoelectricpoint,andlackspotentiallystickycarbohydrates.

ItshouldbenotedthattherelativelyhighmolecularweightofR-PEmayprecludeitsuseinproceduresrequiringgoodpenetrationintocellsandtissues.Itispredominantlyintendedforsurfacelabelingofcellsforflowcytometry.

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