Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of goat IgG heavy chain but not with the Fab portion of goat immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads.Fc fragments and whole IgG molecules have been removed. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for sequential labeling applications.

To complex with primary antibody in solution, use 1:1 weight ratio of Fab:primary antibody (3:1 molar ratio). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay.

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Alexa Fluor® 594

Amax: 591Emax: 614nm

Alexa Fluor® 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Alexa Fluor® 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

This product is provided under an agreement between Life Technologies Corporation and Jackson ImmunoResearch Laboratories, Inc, and the manufacture, use, sale or import of this product is sold pursuant to a license from Life Technologies Corporation for use of its fluorescent dye technology. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell or otherwise transfer this product or its components to any third party, or for any other commercial purposes. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 or outlicensing@lifetech.com.

Jackson公司二抗现货供应Jackson ImmunoResearch公司是美国著名的二抗生产厂商，DyLigh™系列荧光标记是一种近年来被广泛应用的新型荧光染料，主要优点为：具有更好的光谱宽度、更强的荧光强度、更高的光学耐受性（抗淬灭性）、对pH不敏感和分子小而渗透性更好。DylightTM荧光染料激发和发射光谱:荧光染料最大激发波长（nm）最大发射波长（nm）相似染料DyLight 405蓝色400421AlexaTM405DyLight 488绿色493517AlexaTM 488，Cy2®，FITCDyLight 549橙色555568AlexaTM 546，AlexaTM 555，Cy3®，TRITCDyLight 594红色591618AlexaTM594，Texas RedDyLight 649褐红652670AlexaTM647，APC，Cy5® 促销产品价格：货号名称产地规格报价特价/元库存016-470-084DyLight405 Streptavidin 现货，蓝色荧光标记Jackson1MG25921944是016-540-084Alexa Fluor® 488 Streptavidin 绿色荧光标记Jackson1MG25921944是016-600-084Alexa Fluor® 647 Streptavidin  红色荧光 标记Jackson1MG25921944是常用二抗（现货）：100ul为分装酶标二抗：货号名称产地规格报价特价/元115-035-003Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)Jackson100µL200160115-035-003Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)Jackson2.0ml15361152111-035-003Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson100µL200160111-035-003Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson2.0ml15361152305-035-003Peroxidase AffiniPure Rabbit 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IgG (H+L)                 Jackson2.0mg1200900111-095-003FITC-AffiniPure Goat Anti-Rabbit IgG (H+L)             Jackson100µL180144111-095-003FITC-AffiniPure Goat Anti-Rabbit IgG (H+L)             Jackson2.0mg1200900111-165-003Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L)              Jackson100µL200160111-165-003Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L)              Jackson2.0mg15361152111-025-003Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)          Jackson100µL180144111-025-003Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)          Jackson2.0mg1200900111-475-003DyLight 405-AffiniPure Goat Anti-Rabbit IgG (H+L)     Jackson100µL220176111-475-003DyLight 405-AffiniPure Goat Anti-Rabbit IgG (H+L)     Jackson1.5mg16001200111-545-003Alexa Fluor 488 AffiniPure Goat Anti-Rabbit IgG (H+L)    Jackson100µL220176111-545-003Alexa Fluor 488 AffiniPure Goat Anti-Rabbit IgG (H+L)    Jackson1.5mg16001200111-585-003Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (H+L)  Jackson100µL220176111-585-003Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (H+L)  Jackson1.5mg16001200111-605-003Alexa Fluor 647 AffiniPure Goat Anti-Rabbit IgG (H+L)   Jackson100µL220176111-605-003Alexa Fluor 647 AffiniPure Goat Anti-Rabbit IgG (H+L)   Jackson1.5mg16001200711-545-152Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson100µL420336711-545-152Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson0.5mg20801560711-585-152Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson100µL420336711-585-152Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson0.5mg20801560305-095-003FITC-AffiniPure Rabbit Anti-Goat IgG (H+L)            Jackson100µL220176305-095-003FITC-AffiniPure Rabbit Anti-Goat IgG (H+L)            Jackson1.5mg1200900305-165-003Cy3-AffiniPure Rabbit Anti-Goat IgG (H+L)             Jackson100µL250200305-165-003Cy3-AffiniPure Rabbit Anti-Goat IgG (H+L)             Jackson1.5mg15361152305-025-003Rhodamine(TRITC) AffiniPure Rabbit Anti-Goat IgG 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IgG (H+L)Jackson100µL280224705-065-147Biotin-SP AffiniPure Donkey Anti-Goat IgG (H+L)Jackson0.5ml17121284016-030-084Horseradish Peroxidase AffiniPure streptavidin HRP标记链霉亲和素Jackson100µL200160016-030-084Horseradish Peroxidase AffiniPure streptavidin HRP标记链霉亲和素Jackson1.0mg16961272016-050-084Alkaline Phosphatase AffiniPure streptavidin AP标记链霉亲和素Jackson100µL280224016-050-084Alkaline Phosphatase AffiniPure streptavidin AP标记链霉亲和素Jackson1.0mg26882016特别推荐流式二抗（F 片段化抗体）货号名称产地规格报价特价/元115-546-146Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)Jackson100µL420336115-546-146Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)Jackson0.75mg30082256115-116-071PE AffiniPure F(ab')2 Frag Goat Anti-Mouse IgG, Fcγ Frag SpecJackson100µL458367115-116-071PE AffiniPure F(ab')2 Frag Goat Anti-Mouse IgG, Fcγ Frag SpecJackson1.0mg33602520111-546-144Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson100µL420336111-546-144Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson0.75mg33602520111-116-144R-Phycoerythrin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson100µL458367111-116-144R-Phycoerythrin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson1.0mg33602520705-546-147Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)Jackson100µL528396705-546-147Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)Jackson0.3mg24961872705-116-147PE AffiniPure F(ab')2 Frag Donkey Anti-Goat IgG (H+L)Jackson100µL458367705-116-147PE AffiniPure F(ab')2 Frag Donkey Anti-Goat IgG (H+L)Jackson1.0mg16601245常用封闭用血清：货号名称产地规格报价特价(元)017-000-121封闭用正常驴血清Jackson10ml800600011-000-120封闭用正常兔血清Jackson5ml528396015-000-120封闭用正常小鼠血清Jackson5ml704528005-000-121封闭用正常山羊血清Jackson10ml80060010828-028KnockOut™ Serum Replacement血清替代品Invitrogen500ml3300220010099-141Fetal Bovine Serum Australia Origin（胎牛血清，澳大利亚来源）Invitrogen500ml5427255016000-044Fetal Bovine Serum US 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