Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab")₂/Fab portion of human IgG.It also reacts with the light chains of other human immunoglobulins.No antibody was detected against the Fc portion of human IgG or against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applicationsDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Cyanine Cy™3

Amax: 550Emax: 570nm

Cy3 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates. Cy3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy3 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. We recommend Cy3 as a brighter alternative to TRITC. Cy3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy3 has been used with fluorescein for double labeling; however, the use of a narrow band-pass emission filter for fluorescein is recommended to minimize Cy3 fluorescence in the FITC filter set. Cy3 can also be paired with Alexa Fluor® 647 for multiple labeling when using a confocal microscope. However, a better choice for multiple labeling is Rhodamine Red-X because its fluorescence is midway between a green fluorescing dye (like Alexa Fluor® 488) and a far-red-fluorescing dye like Alexa Fluor® 647.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Cy is a trademark of GE Healthcare Bio-Sciences Ltd. Jackson ImmunoResearch is licensed by GE Healthcare Bio-Sciences Ltd. to manufacture and sell conjugates of Cy2, Cy3, and Cy5 reactive dyes under US Patent Number 5,268,486, and other patents pending.

Jackson公司二抗现货供应Jackson ImmunoResearch公司是美国著名的二抗生产厂商，DyLigh™系列荧光标记是一种近年来被广泛应用的新型荧光染料，主要优点为：具有更好的光谱宽度、更强的荧光强度、更高的光学耐受性（抗淬灭性）、对pH不敏感和分子小而渗透性更好。DylightTM荧光染料激发和发射光谱:荧光染料最大激发波长（nm）最大发射波长（nm）相似染料DyLight 405蓝色400421AlexaTM405DyLight 488绿色493517AlexaTM 488，Cy2®，FITCDyLight 549橙色555568AlexaTM 546，AlexaTM 555，Cy3®，TRITCDyLight 594红色591618AlexaTM594，Texas RedDyLight 649褐红652670AlexaTM647，APC，Cy5® 促销产品价格：货号名称产地规格报价特价/元库存016-470-084DyLight405 Streptavidin 现货，蓝色荧光标记Jackson1MG25921944是016-540-084Alexa Fluor® 488 Streptavidin 绿色荧光标记Jackson1MG25921944是016-600-084Alexa Fluor® 647 Streptavidin  红色荧光 标记Jackson1MG25921944是常用二抗（现货）：100ul为分装酶标二抗：货号名称产地规格报价特价/元115-035-003Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)Jackson100µL200160115-035-003Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)Jackson2.0ml15361152111-035-003Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson100µL200160111-035-003Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson2.0ml15361152305-035-003Peroxidase AffiniPure Rabbit 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IgG (H+L)Jackson100µL280224705-065-147Biotin-SP AffiniPure Donkey Anti-Goat IgG (H+L)Jackson0.5ml17121284016-030-084Horseradish Peroxidase AffiniPure streptavidin HRP标记链霉亲和素Jackson100µL200160016-030-084Horseradish Peroxidase AffiniPure streptavidin HRP标记链霉亲和素Jackson1.0mg16961272016-050-084Alkaline Phosphatase AffiniPure streptavidin AP标记链霉亲和素Jackson100µL280224016-050-084Alkaline Phosphatase AffiniPure streptavidin AP标记链霉亲和素Jackson1.0mg26882016特别推荐流式二抗（F 片段化抗体）货号名称产地规格报价特价/元115-546-146Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)Jackson100µL420336115-546-146Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)Jackson0.75mg30082256115-116-071PE AffiniPure F(ab')2 Frag Goat Anti-Mouse IgG, Fcγ Frag SpecJackson100µL458367115-116-071PE AffiniPure F(ab')2 Frag Goat Anti-Mouse IgG, Fcγ Frag SpecJackson1.0mg33602520111-546-144Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson100µL420336111-546-144Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson0.75mg33602520111-116-144R-Phycoerythrin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson100µL458367111-116-144R-Phycoerythrin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson1.0mg33602520705-546-147Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)Jackson100µL528396705-546-147Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)Jackson0.3mg24961872705-116-147PE AffiniPure F(ab')2 Frag Donkey Anti-Goat IgG (H+L)Jackson100µL458367705-116-147PE AffiniPure F(ab')2 Frag Donkey Anti-Goat IgG (H+L)Jackson1.0mg16601245常用封闭用血清：货号名称产地规格报价特价(元)017-000-121封闭用正常驴血清Jackson10ml800600011-000-120封闭用正常兔血清Jackson5ml528396015-000-120封闭用正常小鼠血清Jackson5ml704528005-000-121封闭用正常山羊血清Jackson10ml80060010828-028KnockOut™ Serum Replacement血清替代品Invitrogen500ml3300220010099-141Fetal Bovine Serum Australia Origin（胎牛血清，澳大利亚来源）Invitrogen500ml5427255016000-044Fetal Bovine Serum US 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