Information Serum is lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS), and freeze dried. Serum was collected before August 2019, prior to SARS-CoV-2 pandemic, and tested by ELISA for antibodies to SARS-CoV-2 Spike S1 Receptor Binding Domain (RBD).Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Preservative:None. Product is not sterile, but may be sterile-filtered or a preservative may be added. Suggested Working Concentration or Dilution Range:1:100 - 1:400 for SARS-CoV-2 antibody ELISAs. Dilution should be optimized for assay of interest. Rehydration according to instruction yields 100% serum.Images & References

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* Apparent antibody concentration quantified compared to a standard curve generated with SARS-CoV-2 Spike S1 Antibody (HC2001) – Genscript A02038-100 Figure legend: ELISA for SARS-CoV-2 antibodies from human serum was carried out as described in Amanat et al. (2020) with the following modifications:Coating/capture material: SARS-CoV-2 Spike Protein RBD (Genscript – Z03483-100) diluted to 2 µg/ml in PBS, and 50 µl (2 µg/well) was coated onto an ELISA plate overnight at 4˚C. Plates were blocked with 1% Bovine Serum Albumin (IgG-Free, Protease-Free) (001-000-162) in PBS + 0.05% Tween-20 (PBST). Samples: Human IgG sourced pre-August 2019 [ChromPure Human IgG, whole molecule (009-000-003)] was diluted to 250 µg/ml in 1% BSA/PBST, in triplicate, (100 µl/well). Human Serum sourced pre-August 2019 (009-000-243) was diluted 1:150 in 1% BSA/PBST in triplicate (100 µl/well). Human serum confirmed to be SARS-CoV-2 naive was drawn from seven volunteers. Human serum deemed to be SARS-CoV-2 antibody positive (~ 26-37 days after exposure to SARS-CoV-2, confirmed diagnosis) was drawn from four individuals. Volunteer-derived human serum samples were diluted 1:150 in 1% BSA/PBST (100 µl/well).Detection: HRP-Conjugated secondary antibody (109-035-064) was diluted in PBST at 1:20,000 and added to each well (100 µl/well). Plates were incubated with TMB (Moss TMBHK-1000) for 30 minutes at room temperature. TMB was detected at 620 nm using a plate reader.Standard/calibration curve:Standard curves were generated using Human IgG SARS-CoV-2 Spike S1 Antibody (HC2001) (Genscript A02038). The antibody was serially diluted (1:3) in 1% BSA/PBST, in triplicate, across the ELISA plate starting at 200 ng/ml (100 µl/well).Analysis: Graphpad Prism 8 was used to fit non-linear regression curves to a standard curve generated using the anti-SARS-CoV-2 antibody. Results and apparent antibody concentrations (µg/ml) in serum were interpolated from the curves generated.

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Jackson公司二抗现货供应Jackson ImmunoResearch公司是美国著名的二抗生产厂商，DyLigh™系列荧光标记是一种近年来被广泛应用的新型荧光染料，主要优点为：具有更好的光谱宽度、更强的荧光强度、更高的光学耐受性（抗淬灭性）、对pH不敏感和分子小而渗透性更好。DylightTM荧光染料激发和发射光谱:荧光染料最大激发波长（nm）最大发射波长（nm）相似染料DyLight 405蓝色400421AlexaTM405DyLight 488绿色493517AlexaTM 488，Cy2®，FITCDyLight 549橙色555568AlexaTM 546，AlexaTM 555，Cy3®，TRITCDyLight 594红色591618AlexaTM594，Texas RedDyLight 649褐红652670AlexaTM647，APC，Cy5® 促销产品价格：货号名称产地规格报价特价/元库存016-470-084DyLight405 Streptavidin 现货，蓝色荧光标记Jackson1MG25921944是016-540-084Alexa Fluor® 488 Streptavidin 绿色荧光标记Jackson1MG25921944是016-600-084Alexa Fluor® 647 Streptavidin  红色荧光 标记Jackson1MG25921944是常用二抗（现货）：100ul为分装酶标二抗：货号名称产地规格报价特价/元115-035-003Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)Jackson100µL200160115-035-003Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)Jackson2.0ml15361152111-035-003Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson100µL200160111-035-003Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson2.0ml15361152305-035-003Peroxidase AffiniPure Rabbit 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(H+L)  Jackson1.5mg16001200115-605-003Alexa Fluor 647- AffiniPure Goat Anti-Mouse IgG (H+L) Jackson100µL220176115-605-003Alexa Fluor 647- AffiniPure Goat Anti-Mouse IgG (H+L) Jackson1.5mg16001200115-475-003DyLight 405-AffiniPure Goat Anti-Mouse IgG (H+L)      Jackson100µL220176115-475-003DyLight 405-AffiniPure Goat Anti-Mouse IgG (H+L)      Jackson1.5mg16001200715-545-150Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L)Jackson100µL420336715-545-150Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L)Jackson0.5mg20801560715-585-150Alexa Fluor 594-AffiniPure Donkey Anti-Mouse IgG (H+L)   Jackson100µL420336715-585-150Alexa Fluor 594-AffiniPure Donkey Anti-Mouse IgG (H+L)   Jackson0.5mg20801560112-095-003FITC-AffiniPure Goat Anti-Rat IgG (H+L)                Jackson100µL180144112-095-003FITC-AffiniPure Goat Anti-Rat IgG (H+L)                Jackson2.0mg1200900112-165-003Cy3-AffiniPure Goat Anti-Rat IgG (H+L)                 Jackson100µL200160112-165-003Cy3-AffiniPure Goat Anti-Rat IgG (H+L)                 Jackson2.0mg1200900111-095-003FITC-AffiniPure Goat Anti-Rabbit IgG (H+L)             Jackson100µL180144111-095-003FITC-AffiniPure Goat Anti-Rabbit IgG (H+L)             Jackson2.0mg1200900111-165-003Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L)              Jackson100µL200160111-165-003Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L)              Jackson2.0mg15361152111-025-003Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)          Jackson100µL180144111-025-003Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)          Jackson2.0mg1200900111-475-003DyLight 405-AffiniPure Goat Anti-Rabbit IgG (H+L)     Jackson100µL220176111-475-003DyLight 405-AffiniPure Goat Anti-Rabbit IgG (H+L)     Jackson1.5mg16001200111-545-003Alexa Fluor 488 AffiniPure Goat Anti-Rabbit IgG (H+L)    Jackson100µL220176111-545-003Alexa Fluor 488 AffiniPure Goat Anti-Rabbit IgG (H+L)    Jackson1.5mg16001200111-585-003Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (H+L)  Jackson100µL220176111-585-003Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (H+L)  Jackson1.5mg16001200111-605-003Alexa Fluor 647 AffiniPure Goat Anti-Rabbit IgG (H+L)   Jackson100µL220176111-605-003Alexa Fluor 647 AffiniPure Goat Anti-Rabbit IgG (H+L)   Jackson1.5mg16001200711-545-152Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson100µL420336711-545-152Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson0.5mg20801560711-585-152Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson100µL420336711-585-152Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L)Jackson0.5mg20801560305-095-003FITC-AffiniPure Rabbit Anti-Goat IgG (H+L)            Jackson100µL220176305-095-003FITC-AffiniPure Rabbit Anti-Goat IgG (H+L)            Jackson1.5mg1200900305-165-003Cy3-AffiniPure Rabbit Anti-Goat IgG (H+L)             Jackson100µL250200305-165-003Cy3-AffiniPure Rabbit Anti-Goat IgG (H+L)             Jackson1.5mg15361152305-025-003Rhodamine(TRITC) AffiniPure Rabbit Anti-Goat IgG (H+L)Jackson100µL220176305-025-003Rhodamine(TRITC) AffiniPure Rabbit Anti-Goat IgG (H+L)Jackson1.5mg1200900305-475-003DyLight 405 AffiniPure Rabbit Anti-Goat IgG (H+L)      Jackson100µL280224305-475-003DyLight 405 AffiniPure Rabbit Anti-Goat IgG (H+L)      Jackson1.5mg16001200305-545-003Alexa Fluor 488 AffiniPure Rabbit Anti-Goat IgG (H+L)   Jackson100µL280224305-545-003Alexa Fluor 488 AffiniPure Rabbit Anti-Goat IgG (H+L)   Jackson1.0mg16001200305-585-003Alexa Fluor 594 AffiniPure Rabbit Anti-Goat IgG (H+L)   Jackson100µL280224305-585-003Alexa Fluor 594 AffiniPure Rabbit Anti-Goat IgG (H+L)   Jackson1.0mg16001200305-605-003Alexa Fluor 647 AffiniPure Rabbit Anti-Goat IgG (H+L)   Jackson100µL280224305-605-003Alexa Fluor 647 AffiniPure Rabbit Anti-Goat IgG (H+L)   Jackson1.0mg16001200705-545-147Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H+L) Jackson100µL420336705-545-147Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H+L) Jackson0.5mg20801560705-585-147Alexa Fluor 594 AffiniPure Donkey Anti-Goat IgG (H+L)  Jackson100µL420336705-585-147Alexa Fluor 594 AffiniPure Donkey Anti-Goat IgG (H+L)  Jackson0.5mg20801560313-095-003FITC-AffiniPure Rabbit Anti-Sheep IgG (H+L)           Jackson100µL180144313-095-003FITC-AffiniPure Rabbit Anti-Sheep IgG (H+L)           Jackson1.5mg1200900313-165-003Cy3-AffiniPure Rabbit Anti-Sheep IgG (H+L)            Jackson100µL200160313-165-003Cy3-AffiniPure Rabbit Anti-Sheep IgG (H+L)            Jackson2.0mg15361152SP检测法：货号名称产地规格报价特价/元115-065-146Biotin-SP AffiniPure Goat Anti-Mouse IgG (H+L) Jackson100µL200160115-065-146Biotin-SP AffiniPure Goat Anti-Mouse IgG (H+L) Jackson1.5ml21441608112-065-143Biotin-SP AffiniPure Goat Anti-Rat IgG (H+L)    Jackson100µL200160112-065-143Biotin-SP AffiniPure Goat Anti-Rat IgG (H+L)    Jackson1.5ml21441608111-065-144Biotin-SP AffiniPure Goat Anti-Rabbit IgG (H+L)Jackson100µL200160111-065-144Biotin-SP AffiniPure Goat Anti-Rabbit IgG (H+L)Jackson1.5ml21441608705-065-147Biotin-SP AffiniPure Donkey Anti-Goat IgG (H+L)Jackson100µL280224705-065-147Biotin-SP AffiniPure Donkey Anti-Goat IgG (H+L)Jackson0.5ml17121284016-030-084Horseradish Peroxidase AffiniPure streptavidin HRP标记链霉亲和素Jackson100µL200160016-030-084Horseradish Peroxidase AffiniPure streptavidin HRP标记链霉亲和素Jackson1.0mg16961272016-050-084Alkaline Phosphatase AffiniPure streptavidin AP标记链霉亲和素Jackson100µL280224016-050-084Alkaline Phosphatase AffiniPure streptavidin AP标记链霉亲和素Jackson1.0mg26882016特别推荐流式二抗（F 片段化抗体）货号名称产地规格报价特价/元115-546-146Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)Jackson100µL420336115-546-146Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)Jackson0.75mg30082256115-116-071PE AffiniPure F(ab')2 Frag Goat Anti-Mouse IgG, Fcγ Frag SpecJackson100µL458367115-116-071PE AffiniPure F(ab')2 Frag Goat Anti-Mouse IgG, Fcγ Frag SpecJackson1.0mg33602520111-546-144Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson100µL420336111-546-144Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson0.75mg33602520111-116-144R-Phycoerythrin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson100µL458367111-116-144R-Phycoerythrin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)Jackson1.0mg33602520705-546-147Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)Jackson100µL528396705-546-147Alexa Fluor® 488-AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)Jackson0.3mg24961872705-116-147PE AffiniPure F(ab')2 Frag Donkey Anti-Goat IgG (H+L)Jackson100µL458367705-116-147PE AffiniPure F(ab')2 Frag Donkey Anti-Goat IgG (H+L)Jackson1.0mg16601245常用封闭用血清：货号名称产地规格报价特价(元)017-000-121封闭用正常驴血清Jackson10ml800600011-000-120封闭用正常兔血清Jackson5ml528396015-000-120封闭用正常小鼠血清Jackson5ml704528005-000-121封闭用正常山羊血清Jackson10ml80060010828-028KnockOut™ Serum Replacement血清替代品Invitrogen500ml3300220010099-141Fetal Bovine Serum Australia Origin（胎牛血清，澳大利亚来源）Invitrogen500ml5427255016000-044Fetal Bovine Serum US 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