The EpiQuik™ Dnmt1 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt1 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt1 assay experiment without the need for electrophoresis and transfer process. The EpiQuik™ Dnmt1 Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse. The kit has the following advantages:

• The fastest procedure, which can be finished within 3 hours.
• Innovative colorimetric assay to quantitatively measure Dnmt1 amount without the need for electrophoresis.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

Principle & ProcedureThe EpiQuik™ Dnmt1 Assay Kit is designed for measuring total Dnmt1 amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt1 contained in the sample binds to the substrate. The bound Dnmt1 can be recognized with a specific Dnmt1 antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt1 is proportional to the intensity of color development.

Fig. 1. Schematic procedure for using the EpiQuik Dnmt1 Assay Kit.

Fig. 2.Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.