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蚂蚁淘在线 / 品牌中心 / Epigentek / Epigentek/MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)/P-1039-96/96 assays

Epigentek/MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)/P-1039-96/96 assays

价格
￥10180.00
货号：P-1039-96
浏览量：127
品牌：Epigentek
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商品描述
InputType:Urine
ResearchArea:DNAMethylation
TargetApplication:AmountQuantitation
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheMethylFlash™Urine5-Methylcytosine(5-mC)QuantificationKit (Colorimetric)  isacompletesetofoptimizedbuffersandreagentstocolorimetricallyquantify5-mCinurineusinganinhibitorycompetitiveimmunoassaymethod.It issuitablefordetectingtotalurinary5-methylcytosinelevels,resultingfromwholebodyturnoverordegradationofmethylatedDNA/RNA,inurinefromhumansandanimals.Theurinesamplescanbeinfreshorfrozenform. Thekithasthefollowingadvantagesandfeatures:

  • Innovativecolorimetricassaywitheasy-to-followstepsforconvenienceandspeed.Theentireprocedurecanbefinishedwithin4hours.
  • 96strip-wellmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
  • Innovativekitcompositionenablesbackgroundsignalstobeextremelylow,whicheliminatestheneedforplateblockingandallowstheassaytobesimple,accurate,reliable,andconsistent.
  • Thelevelof5-mCmeasuredinhumanurinesamplesusingthiskitiscomparabletothatdetectedbyHPLCmethod. 
  • Anovelassayprincipleallowshighsensitivitytobeachieved.Thedetectionlimitcanbeaslowas0.03ng/assaywellor1nMof5-mC.
  • Lowinputrangeofurineforeachassaywithavolumeof0.5to5µlandanoptimalvolumeof1µl.
  • Optimizedantibodyandenhancersolutionsallowforhighspecificityto5-mC,withoutcross-reactivitytounmethylatedcytosine. 
  • Negativecontrolandpositivestandardareincluded,whicharesuitableforquantificationof5-mCinfreeformand5-mCcontainedinmethylatedDNA/RNAfragmentsfromdifferenturinesamples.

BackgroundInformation
Nucleobase5-methylcytosine(5-mC),amodifiedformofcytosineconvertedbycytosinemethyltransferases,iswidespreadbothinDNAanddifferentcellularRNAs.TheBIOLOGicalimportanceofDNA5-mCmethylationasamajorepigeneticmodificationinphenotypeandgeneexpressionhasbeenwidelyrecognized.RecentdatastronglysuggeststhatRNA5-mCmethylationisalsoinvolvedintheregulationofvariousbiologicalprocessesincludingtRNAstABIlity/recognitionandmRNAtranslation. 

Urinaryexcretionof5-mCincludingboth5-methyl-2-deoxycytidineand5-methylcytidineisanindicationofawholebodyturnoverordegradationofmethylatedDNAandRNA.Theurinary5-mClevelcanbealteredbyachangeofthebodies’turnoverofmethylatedDNA/RNAoralterationofcellularDNA/RNAmethylationstatus.Anumberofstudieshaveindicatedthat5-mCexcretedinurinehasthepotentialtoactasacancerbioMarker,withanincreasedlevelarisingfromdiseaseonsetandprogression.Forexample,anelevatedlevelofurinary5-mCwasobservedinlungcancer,breastcancer,andleukemiapatientswithactivediseasestates.Itwasalsoshownthaturinary5-mCexcretioniseffectedbyAlzheimer’sdiseaseorbyrADIationtreatment.Itissuggestedthaturinary5-mCmightbeapplicableasabiologicalmarkerfordetectingsometypesofcancerandmonitoringcancerprogressionaftertreatmentwithradiationordemethylationreagents. 

Chromatography-basedtechniquessuchasHPLCandTLCmassspectrometryarecommonlyusedfordetecting5-mCinurineandalthoughfairlyacurate,aretimeconsuming,lesssensitive,andhavelowthroughputwithhighcosts.Toaddressthisproblem,EpigentekofferstheMethylFlash™ Urine5-Methylcytosine(5-mC)QuantificationKit (Colorimetric) toquantify5-mCorbodyturnoverstatusofmethylatedDNA/RNAusingurinesamples.

Principle&Procedure
InthisELISA-likeinhibitorycompetitiveimmunoassayassay,urinesamplesandthe5-mCstandardarefirstincubatedwitha5-mCantibodysolutionandthentransferredtostripwellscoatedwithmethylatedDNA(5-mC).Thewelliswashedtoremoveanyunboundreagentsafterincubationandthenadetectionantibodyisaddedtogenerateasignalthatcanbemeasuredcolorimetricallybyreadingtheabsorbanceinamicroplatespectrophotometer.Because5-mCintheurinesampleinhibitsthebindingof5-mCantibodyto5-mCcoatedonthewell,higherconcentrationsof5-mCintheurinesampleleadtoareducedbindingoftheantibodytothe5-mConthewell.ThereforethesignalorODintensitymeasuredfromthewellwillbeinverselyproportionaltotheamountof5-mCintheurinesampleandtheamountof5-mCintheurinesamplecanbequantifiedbyacomparisonwithapredetermined5-mCstandard.


Fig.1.SchematicprocedurefortheMethylFlash™Urine5-MethylcytosineQuantificationKit(Colorimetric).


Fig.2. 5-mCstandardwasaddedintotheassaywellsatdifferentconcentrationsandthenmeasuredwiththeMethylFlash™Urine5-MethylcytosineQuantificationKit (Colorimetric).


Fig.3. 5-mClevelisquantifiedfromdifferentvolumesofhumanurineusingtheMethylFlash™Urine5-MethylcytosineQuantificationKit (Colorimetric).

 

 

ProductComponents

WB(10XWashBuffer)
BS(BindingSolution)
MS(5-mCStandard,4µg/ml)*
ME3(NegativeControl,50µg/ml)*
ME4(5-mCDNAsolution,320X)*
ME5(CaptureAntibody,1000X)*
ME6(DetectionAntibody,2000X)*
ES(EnhancerSolution)*
DS(DeveloperSolution)
SS(StopSolution)
Plate1(AssayPlate)(WithFrame)
Plate2(SamplePreparationPlate)
UserGuide

*Spinthesolutiondowntothebottompriortouse.

 

美国Epigentek集团公司是全球领先的表观遗传学相关研究的技术创新者和产品开发供应商。公司开发了700多个专利产品，为表观遗传学方面的研究和新药研发提供全面系统的解决方案。相比普通方法的产品而言，这些独特的产品在科研分析过程中更简单、更快捷、更方便、更有效。全世界的备受推崇的学者、领先的制药企业以及先进的生物技术公司都在使用Epigentek产品。让客户满意和帮助客户在试验中取得最大成功是我们进行技术创新的首要任务。Epigentek products are used all around the world by esteemed academia, leading pharmaceuticals, and cutting-edge biotechnology companies. These unique products are specifically designed to make assays much simpler, faster, more convenient, and highly more efficient than currently used methods. Epigentek's products are sold through a dedicated sales force and a global network of distributors in more than thirty six countries and regions. It is our number one priority to ensure that the innovation of our technology will result in complete customer satisfaction and the greatest success in your experiments. No.货号规格EpigentekEpigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)2A-9001-100100 µgEpigentekAnnexin V Monoclonal Tag Antibody [VAA-33]100 µgEpigentekBiotin Monoclonal Tag Antibody [B25]5A26001-1100 µgEpigentekc-Myc Polyclonal Tag Antibody, FITC Conjugated7A26402-1100 µlEpigentekEpiQuik Circulating Modified Histone H3 Multiplex Assay Kit (Colorimetric)9P-3091-9696 assaysEpigentekEpiQuik Circulating Trimethyl Histone H3K4 ELISA Kit (Colorimetric)11P-3116-9696 assaysEpigentekAATF Polyclonal Antibody13A5167650ugEpigentekABHD14B Polyclonal Antibody15A5738050ug