Technical Documents
Description
Rhodamine derivatives are not sufficiently stable to survive conventional deprotection and these must be attached to amino-modified oligonucleotides using post-synthesis labelling techniques. Because Tetramethyl Rhodamine (TAMRA) is not base stable, the procedure to cleave and deprotect the labelled oligonucleotide must be carefully considered. Using the UltraMILD monomers and deprotection with potassium carbonate in methanol, TAMRA oligonucleotides can be fairly conveniently isolated. To streamline the preparation of TAMRA oligos, we offer 3"-TAMRA CPG for 3" labelling and TAMRA-dT for labelling within the sequence. We also offer TAMRA NHS ester for labelling amino-modified oligonucleotides.
The spectral characteristics of this dye is detailed here.
Details
Usage
- Coupling: Conjugate with an amino-modified oligo in sodium carbonate/bicarbonate buffer (pH=9).See Technical Bulletin for details.
- Deprotection: not applicable
| Specifications | |
|---|---|
| Diluent | ~0.17M Solution in Anhydrous DMSO |
| Storage | Freezer storage, -10 to -30°C, dry |
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Product FAQs
- What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
- What are the relative extinction coefficients of various dyes?
- Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?
- Dye Quencher Plot
