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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
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https://www.ebiomall.com
商品描述
| Description | ATPindependentT4RNALigase2specificallyligatesthepreadenylated5´endofDNAorRNAtothe3´OHendofRNA.TheenzymedoesnotrequireATPforligationbutrequirespre-adenylatedlinkers. |
| Applications |
|
| Source | PurifiedfromarecombinantE.colistraincarryingamutantofT4RNALigase2. |
| Components |
|
| UnitDefinition | 200unitsisdefinedastheamountofenzymerequiredtogive80%ligationofa31-merRNAtothepre-adenylatedendofa17-merDNAinatotalreactionvolumeof20μlin1hourat25°C. 5´-FAM-rArGrUrCrGrUrArGrCrCrUrUrUrArUrCrCrGrArGrArUrUrCrArGrCrArArUrA-3´ |
| QualityControl | Theabsenceofendo-,exodeoxyribonucleases,phosphataseandribonucleasesconfirmedbyappropriatequalitytests. |
| Storage | -20°C. |


