| Description | Gst DNA Polymerase is functionally equivalent to the large fragment of Bsm Polymerase I and Bst Polymerase I. Recombinant Gst DNA Polymerase (large fragment) has 5’-> 3’ polymerase activity, but lacks 5’->3’ exonuclease and 3\'→5\' exonuclease activities. Gst DNA Polymerase, Large Fragment, has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications such as rapid amplification of trace amounts of DNA templates for sequencing and LAMP. |
| Applications | - Isothermal DNA amplification by the method of:
- Loop-mediated isothermal amplification (LAMP).
- Whole genome amplification (WGA).
- Ramification amplification (RAM).
- Random-primed DNA labeling
- Labeling by fill-in 5\'-overhangs of dsDNA
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| Source | A recombinant E. coli strain carrying the large fragment of DNA polymerase gene from Geobacillus stearothermophilus |
| Unit Definition | One unit is defined as the amount of polymerase required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C. |
| Components | - Gst DNA Polymerase: 100,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C
- 10X Reaction Buffer: 200 mM Tris-HCl, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100, pH 8.8 @ 25°C
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| Quality Control | The absence of endonuclease, nicking activity and exonuclease activity is confirmed by appropriate quality tests. |
| Storage Condition | -20 °C |
