| Description | Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, is a homolog of Escherichia coli exonuclease III. APE1 acts as an AP lyase by hydrolyzing the phosphodiester backbone at the 5′ end of an apurinic (AP) site, generating a single-strand DNA break in the DNA duplex and leaving 3′-hydroxyl and 5′-deoxyribose phosphate termini. It is reported that APE 1 may also exhibit weak DNA 3′-diesterase, 3′ to 5′ exonuclease and RNase H activities. |
| Applications | - Single cell gel electrophoresis (Comet assay)
- Alkaline elution
- Alkaline unwinding
- Modified nick translation
|
| Source | An E. coli strain which carries the cloned human APE 1 gene. |
| Unit Definition | One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34-mer oligonucleotide duplex contain-ing a single AP site in 1 hour at 37°C |
| Components | - APE 1: 10,000 units/ml in 10 mM Tris-HCl, pH 8.0 @ 25°C, 50 mM NaCl, 1 mM DTT, 0.05 mM EDTA, 200 μg/ml BSA, 50% Glycerol
- 10X Reaction Buffer: 200 mM Tris-Acetate, 500 mM Potassium Acetate, pH 7.9 @ 25°C, 10 mM Dithiothreitol, 100 mM Magnesium Acetate.
|
| Quality Control | The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functional test. |
| Storage Condition | -20 °C |
