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Millipore/MAB388-100UG | Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1/MAB388-100UG/100 µg

价格
¥7400.00
货号:MAB388-100UG
浏览量:127
品牌:Millipore
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商品描述
Description
CatalogueNumberMAB388-100UG
ReplacesMAB388
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-MyelinProteolipidProteinAntibody,CT,clonePLPC1
AlternateNames
  • PLP
ProductInformation
FormatPurified
Control
  • Brain
PresentationPurifiedmouseimmunoglobulininPBS,pH7.2containing0.09%sodiumazide.
StorageandShippingInformation
StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Applications
ApplicationDetectMyelinProteolipidProteinusingthisAnti-MyelinProteolipidProteinAntibody,C-terminus,clonePLPC1validatedforuseinIH,IH(P)&WB.
KeyApplications
  • Immunohistochemistry
  • Immunohistochemistry(Paraffin)
  • WesternBlotting
ApplicationNotesWesternBlotting:detectsa23-25kDaproteinandDM20a20kDaprotein;PLPcanaggregatecausingHMWbandstosometimesappearincertainpreparations.

Immunocytochemistry:4%PFAfixedcells,5-10minutesroomtemperature.Use0.1%tritoninblockonlytoallowantibodyaccesstoC-terminalepitope.

Immunohistologyonfreshfrozensectionsor4%PFAfixed,frozentissues.Epitopeisinternal,use0.1-0.2%tritonX-100intheblockingstepforpermeABIlization.

Immunohistochemistryinparaffin:worksbestwithlightlyfixedtissues,orBouin"sfixedmaterial,althoughtherearereportsoftrADItionalformalinfixationalsoworkingwithantigenrecovery.{Mathis,etal2003}.

Optimalworkingdilutionsmustbedeterminedbyenduser.
BIOLOGicalInformation
ImmunogenMyelinproteolipidprotein,syntheticpeptideCGRGTKFcorrespondingtotheC-terminalpeptide.
EpitopeC-terminus
ClonePLPC1
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostMouse
SpecificityReactswithmyelinproteolipidproteininmanymammalianspecies.Showscross-reactivitytoDM20.
IsotypeIgG2a
SpeciesReactivity
  • Mammals
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesatransmembraneproteolipidproteinthatisthepredominantmyelinproteinpresentinthecentralnervoussystem.Itmayplayaroleinthecompaction,stabilization,andmaintenanceofmyelinsheaths,aswellasinoligodendrocytedevelopmentandaxonalsurvival.MutationsinthisgenecauseX-linkedPelizaeus-Merzbacherdiseaseandspasticparaplegiatype2.Alternativelysplicedtranscriptvariantsencodingdistinctisoformsorhavingdifferent5"UTRs,havebeenidentifiedforthisgene.
GeneSymbol
  • PLP
  • PMD
  • MMP
  • SPG2
  • PLP/DM20
PurificationMethodProteinAPurfied
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P60201#Thisisthemajormyelinproteinfromthecentralnervoussystem.Itplaysanimportantroleintheformationormaintenanceofthemultilamellarstructureofmyelin.
SIZE:277aminoacids;30,077Da
SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein.
PTM:Lipoprotein,Palmitate
DISEASE:DefectsinPLP1arethecauseofPelizaeus-Merzbacherdisease(PMD)[MIM:312080].PMDisanX-linkedneurologicdisorderofmyelinmetabolism.Itischaracterizedbyearlyimpairmentofmotordevelopment(duringthefirstthreemonthsoflife)and,later,bythedevelopmentofabnormalmovementsandprogressivespasticparaplegia.
SIMILARITY:Belongstothemyelinproteolipidproteinfamily.
MolecularWeight23-25kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
Millipore 提供三种不同的 Immobilon PVDF 膜,是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比,Immobilon 膜具有多种优点。 它们不易破裂或卷 曲,便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外,它们可以重复检测。 Immobilon-P 膜(沃比森-供应) ·         0.45μm 孔径 ·         建议用于大多数免疫印迹,特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间,将检测时间至少减少了两个小时,而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时,印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜,其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜(沃比森-供应) ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜(沃比森-供应) ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针(所有激发和发射波长)兼容 ·         是多态性检测和化学荧光应用的理想选择