Description
CatalogueNumber	MAB388-100UG
Replaces	MAB388
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-MyelinProteolipidProteinAntibody,CT,clonePLPC1
AlternateNames	PLP

ProductInformation
Format	Purified
Control	Brain
Presentation	PurifiedmouseimmunoglobulininPBS,pH7.2containing0.09%sodiumazide.

StorageandShippingInformation
StorageConditions	Maintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

Applications
Application	DetectMyelinProteolipidProteinusingthisAnti-MyelinProteolipidProteinAntibody,C-terminus,clonePLPC1validatedforuseinIH,IH(P)&WB.
KeyApplications	Immunohistochemistry Immunohistochemistry(Paraffin) WesternBlotting
ApplicationNotes	WesternBlotting:detectsa23-25kDaproteinandDM20a20kDaprotein;PLPcanaggregatecausingHMWbandstosometimesappearincertainpreparations. Immunocytochemistry:4%PFAfixedcells,5-10minutesroomtemperature.Use0.1%tritoninblockonlytoallowantibodyaccesstoC-terminalepitope. Immunohistologyonfreshfrozensectionsor4%PFAfixed,frozentissues.Epitopeisinternal,use0.1-0.2%tritonX-100intheblockingstepforpermeABIlization. Immunohistochemistryinparaffin:worksbestwithlightlyfixedtissues,orBouin"sfixedmaterial,althoughtherearereportsoftrADItionalformalinfixationalsoworkingwithantigenrecovery.{Mathis,etal2003}. Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	Myelinproteolipidprotein,syntheticpeptideCGRGTKFcorrespondingtotheC-terminalpeptide.
Epitope	C-terminus
Clone	PLPC1
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	Reactswithmyelinproteolipidproteininmanymammalianspecies.Showscross-reactivitytoDM20.
Isotype	IgG2a
SpeciesReactivity	Mammals
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_000533.3
EntrezGeneSummary	Thisgeneencodesatransmembraneproteolipidproteinthatisthepredominantmyelinproteinpresentinthecentralnervoussystem.Itmayplayaroleinthecompaction,stabilization,andmaintenanceofmyelinsheaths,aswellasinoligodendrocytedevelopmentandaxonalsurvival.MutationsinthisgenecauseX-linkedPelizaeus-Merzbacherdiseaseandspasticparaplegiatype2.Alternativelysplicedtranscriptvariantsencodingdistinctisoformsorhavingdifferent5"UTRs,havebeenidentifiedforthisgene.
GeneSymbol	PLP PMD MMP SPG2 PLP/DM20
PurificationMethod	ProteinAPurfied
UniProtNumber	P60201
UniProtSummary	FUNCTION:SwissProt:P60201#Thisisthemajormyelinproteinfromthecentralnervoussystem.Itplaysanimportantroleintheformationormaintenanceofthemultilamellarstructureofmyelin. SIZE:277aminoacids;30,077Da SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein. PTM:Lipoprotein,Palmitate DISEASE:DefectsinPLP1arethecauseofPelizaeus-Merzbacherdisease(PMD)[MIM:312080].PMDisanX-linkedneurologicdisorderofmyelinmetabolism.Itischaracterizedbyearlyimpairmentofmotordevelopment(duringthefirstthreemonthsoflife)and,later,bythedevelopmentofabnormalmovementsandprogressivespasticparaplegia. SIMILARITY:Belongstothemyelinproteolipidproteinfamily.
MolecularWeight	23-25kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择