Description
CatalogueNumber	AB1620
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-VimentinAntibody

ProductInformation
Format	Serum
Control	Breastcarcinomatissue,Colon,Prostate,Tonsil,Tonsilarlymphoma,Uterus,Ureter
Presentation	NeatgoatSerum.Liquidcontaining0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Maintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

Applications
Application	Anti-VimentinAntibodydetectslevelofVimentin&hasbeenpublished&validatedforuseinIC,IH,IH(P)&WB.
KeyApplications	Immunocytochemistry Immunohistochemistry Immunohistochemistry(Paraffin) WesternBlotting
ApplicationNotes	Immunohistochemistry:1:20-1:40.Showsreactivityonfrozentissuesectionsaswellasformalin-fixedparaffinembeddedtissuebyimmunoperoxidaseandimmunofluorescentassays. Immunocytochemistry Westernblot Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	Vimentinpurifiedfromculturedhumanforeskinfibroblasts.
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Goat
Specificity	Recognizesvimentininculturedmammaliancellsandtissuesections.AB1620specificallystainsthevimentinband(58kD)usingtotalcellortissueextractsinimmunoblotting.Noreactivitytodesmin,keratin,neurofilamentsorglialfilaments.Showswidecross-reactivityamongmammalianspeciesusingvariousimmunolabelingtechniques.
SpeciesReactivity	Human Mammals
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_003380.2
EntrezGeneSummary	AlongwiththeMICROFILaments(actins)andmicrotubules(tubulins),theintermediatefilamentsrepresentathirdclassofwell-characterizedcytoskeletalelements.Thesubunitsdisplayatissue-specificpatternofexpression.Desmin(MIM125660)isthesubunitspecificformuscleandvimentinthesubunitspecificformesenchymaltissue.[suppliedbyOMIM]
GeneSymbol	VIM FLJ36605 Vimentin. vimentin
PurificationMethod	Unpurified
UniProtNumber	P08670
UniProtSummary	FUNCTION:SwissProt:P08670#Vimentinsareclass-IIIintermediatefilamentsfoundinvariousnon-epithelialcells,especiallymesenchymalcells. SIZE:466aminoacids;53652Da SUBUNIT:Homopolymer.ComponentofacomplexcomposedatleastofACTB,AP2M1,AP2A1,AP2A2,MEGF10andVIM.InteractswithHCVcoreprotein. TISSUESPECIFICITY:Highlyexpressedinfibroblasts,someexpressioninT-andB-lymphocytes,andlittleornoexpressioninBurkitt"slymphomacelllines.Expressedinmanyhormone-independentmammarycarcinomacelllines. PTM:Oneofthemostprominentphosphoproteinsinvariouscellsofmesenchymalorigin.Phosphorylationisenhancedduringcelldivision,atwhichtimevimentinfilamentsaresignificantlyreorganized. SIMILARITY:SwissProt:P08670##Belongstotheintermediatefilamentfamily.
MolecularWeight	58kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择