Description
CatalogueNumber	MABN681
Description	Anti-TrkAAntibody,clone6B2
AlternateNames	Highaffinitynervegrowthfactorreceptor Neurotrophictyrosinekinasereceptortype1 TRK1-transformingtyrosinekinaseprotein Tropomyosin-relatedkinaseA Tyrosinekinasereceptor TyrosinekinasereceptorA Trk-A gp140trk p140-TrkA
BackgroundInformation	TrkA,apparentmolecularweight140kDa,isahighaffinitynervegrowthfactor(NGF).TheTrkproto-oncogenefamilycontainsfourmembers,TrkA,TrkB,TrkC,andTrkE,whicharevariablyexpressedthroughoutthecentralandperipheralnervoussystems.TrkAbindstonervegrowthfactor(NGF)andautophosphorylatesontyrosineresidues(Tyr490,Tyr674,Tyr675,Tyr751andTyr785)toactivatemultipledownstreameffectorproteins.PhosphorylationatTyr490isrequiredforShcassociationandsubsequentactivationoftheRas-MAPkinase-signalingcascade,whichleadstoactivationofElk-1-dependentgenetranscriptionandneuritegrowth.PhosphorylationsatTyr674andTyr675liewithinthecatalyticdomainofTrkAtyrosinekinaseandreflectTrkkinaseactivity.Additionally,phosphorylationatTyr751isrequiredforPI3-kinaseassociationandactivationoftheAktsignalingcascade.

ProductInformation
Format	Ascites
Control	Humanbraintissuelysate
Presentation	MousemonoclonalIgG2basciticfluidcontainingupto0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.

Applications
Application	Anti-TrkAAntibody,clone6B2isahighlyspecificmousemonoclonalantibody,thattargetsTrkA&hasbeentestedinwesternblotting,IHC&ICC.
KeyApplications	WesternBlotting Immunohistochemistry Immunocytochemistry
ApplicationNotes	ImmunohistochemistryAnalysis:A1:100dilutionfromarepresentativelotdetectedTrkAinhumanbrainandratcerebellumtissues. ImmunocytochemistryAnalysis:A1:200-1,000dilutionfromarepresentativelotdetectedTrkAinPC-12cells. Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	PurifiedrecombinantextracellularfragmentofTrkAexpressedinHEK293cells.
Clone	6B2
Host	Mouse
Isotype	IgG1
SpeciesReactivity	Human Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_002520
GeneSymbol	NTRK1 MTC TRK TRKA
PurificationMethod	Unpurified
UniProtNumber	P04629
MolecularWeight	~87kDaobserved.Uncharacterizedbandsmayappearinsomelysate(s).Uniprotdescribes4isoformsrangingbetween~77kDaand~87kDa.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择