Description
CatalogueNumber	MABD99
Description	Anti-Nkx2.2Antibody,clone1C11
AlternateNames	HomeoboxproteinNkx-2.2 HomeoboxproteinNK-2homologB
BackgroundInformation	HomeoboxproteinNkx2.2isaNK2familytranscriptionfactorthatcontainsa60AA(128to187)DNAbindinghomeobox.Nkx2.2ispartofahomeodomainproteincombinatorialcodethatspecifiesProgenitorcellidentityandneuronalfateintheventralneuraltube.Nkx2-2isrequiredfortheestablishmentofthedopaminergicprogenitordomaininmammalianventralmidbrainandSEROtonergic(5-HT)neuronsinthebrainstemandventralspinalcord.Nkx2.2isselectivelyexpressedinoligodendrocyteprogenitors(OPC)thatarisefromglialprecursorslocatedintheventralneuraltube.StudieshaveshownNkx2.2isakeyregulatorofoligodendrocytedifferentiationbyregulatingdifferentiationand/ormaturation,butnottheinitialspecificationofoligodendrocyteprogenitors.

ProductInformation
Format	Purified
Control	MouseE13-14braintissuelysate
Presentation	PurifiedmousemonoclonalIgG2aκinbuffercontainingPBSwith0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8°Cfromdateofreceipt.

Applications
Application	Anti-Nkx2.2Antibody,clone1C11isahighlyspecificmousemonoclonalantibody&thattargetsNKX2.2&hasbeentestedinwesternblotting.
KeyApplications	WesternBlotting

BIOLOGicalInformation
Immunogen	RecombinantproteincorrespondingtohumanNkx2.2.
Clone	1C11
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Isotype	IgG2aκ
SpeciesReactivity	Mouse
SpeciesReactivityNote	DemonstratedtoreactwithMouse. PredictedtoreactwithHumanbasedonimmunogendesign.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_002500
EntrezGeneSummary	Theproteinencodedbythisgenecontainsahomeoboxdomainandmaybeinvolvedinthemorphogenesisofthecentralnervoussystem.Thisgeneisfoundonchromosome20nearNKX2-4,andthesetwogenesappeartobeduplicatedonchromosome14intheformofTITF1andNKX2-8.Theencodedproteinislikelytobeanucleartranscriptionfactor.[providedbyRefSeq,Jul2008].
GeneSymbol	NKX2-2 NKX2.2 NKX2B
PurificationMethod	ProteinGPurified
UniProtNumber	O95096
UniProtSummary	FUNCTION:Actsasatranscriptionalactivator.RequiredforthemaintenanceofNEUROD1expressioninthehoromone-producingendocrinecellsofthepancreas.Maybeinvolvedinspecifyingdiencephalicneuromericboundaries,andincontrollingtheexpressionofgenesthatplayaroleinaxonalguidance.AssociateswithchromatinattheNEUROD1promoterregion.BindstoasubsetofconsensuselementswithintheNEUROD1promoter(Bysimilarity). SUBUNITSTRUCTURE:InteractswithOLIG2(Bysimilarity). SUBCELLULARLOCATION:Nucleus(Bysimilarity). DOMAIN:ThehomeodomainisessentialforinteractionwithOLIG2(Bysimilarity). SEQUENCESIMILARITIES:BelongstotheNK-2homeoboxfamily. Contains1homeoboxDNA-bindingdomain.
MolecularWeight	~37kDaobserved. Uncharacterizedbandsmayappearat~25kDaand~300kDainsomelysates.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择