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商品描述
AlternativeNameMT3,MT-3
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
BioassayTestedBioassayTested
Everylotistried&testedinarelevantBIOLOGicalassay.
OurBioassay
OriginNaturalproteinisolatedfromDendroaspisangusticeps (Easterngreenmamba)(Najaangusticeps).
MW:7379Da.
Purity:>99%(HPLC)
FormLyophilizedpowder.
Effectiveconcentration100-1000nM.
SequenceLTCVTKNTIFGITTENCPAGQNLCFKRWHYVIPRYTEITRGCAATCPIPENYDSIHCCKTDKCNE.
ModificationsDisulfidebondsbetweenCys3-Cys24,Cys17-Cys42,Cys46-Cys57andCys58-Cys63.
MolecularformulaC319H489N89O97S8.
ActivityMuscarinicreceptor4andadrenoceptorantagoNIST1-4.
- Jolkkonen,M. etal. (1994) FEBSLett. 352, 91.
- Karlsson,E. etal. (1994) Ann.N.Y.Acad.Sci. 710, 153.
- Caulfield,M.P.andBirdsall,N.J. (1998) Pharm.Rev. 50, 279.
- Nareoja,K. etal.(2011) Br.J.Pharmacol. 164, 538.
AccessionnumberP81031.
ShippingandstorageShippedatroomtemperature.Productassuppliedcanbestoredintactatroomtemperatureforseveralweeks.Forlongerperiods,itshouldbestoredat-20°C.
SolubilityAnyaqueousbuffer.Centrifugeallproductpreparationsbeforeuse(10000xg5min).
StorageofsolutionsUptotwoweeksat4°Corthreemonthsat-20°C.
AlomoneLabs MuscarinicToxin3 inhibitsMuscarinicReceptor4expressedinCHO-K1cells.CHO-K1cellswereco-transfectedwithhumanM4andGα15 inordertocoupletheM4receptortotheCa2+ signalingpathway.Ca2+ concentrationsweremonitoredusingFLIPR.Cellswerefirstincubatedinthepresenceofdifferentconcentrationsof MuscarinicToxin3 (#M-140)followedbyOxotremorine.ThepercentageofM4receptorinhibitionwasplottedversusdifferentconcentrationsofMuscarinicToxin3.TheIC50 valueofMuscarinicToxin3wasbetween100to1000nM.
References-Scientificbackground
- 1.Bradley,K.N. (2000) Pharmacol.Ther. 85, 87.
- 2.Nareoja,K. etal.(2011) Br.J.Pharmacol. 164, 538.
- Muscarinictoxinsareisolatedfromthevenomofthemambaspeciesandarecomposedof65to66aminoacidresidueswithfourintramoleculardisulfidebridges.Oneoftheseismuscarinictoxin3(MT-3)whichwasisolatedfromthegreenmamba(Dendroaspisaugusticeps)andiscomposedof65aminoacidresidues1.ArecentstudyinvestigatedtheinteractionofMT3withclonedreceptors(mAChRsandadrenoceptors)expressedininsectcellsbyrADIoligandbinding.MT3appearstohaveabroadspectrumoftargetsshowinghigh-affinitybinding(IC50=1-10nM)toM4mAChR,α1A-,α1D-andα2A-adrenoceptorsandloweraffinitybinding(IC50>25nM)toα1B-andα2C-adrenoceptorsandM1mAChR2.
TargetM1,M4muscarinicreceptors,adrenoceptors
NetPeptideContent:100%
LyophilizedPowder
Clickheretoreceivea50µgfreetrialsample!
Lastupdate:04/11/2019
MuscarinicToxin3(#M-140) isahighlypure,natural,andbiologicallyactivepeptidetoxin.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
