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商品描述
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
BioassayTestedBioassayTested
Everylotistried&testedinarelevantBIOLOGicalassay.
OurBioassay
SourceSynthetic
MW:356.3
Purity:>98%(HPLC)
FormLyophilizedpowder.
Effectiveconcentration50nM-5µM.
Chemicalname(4S)-1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-trifluoromethyl)phenyl]-3-pyridinecarboxylicacidmethylester.
MolecularformulaC16H15 F3N2O4.
CASNo.:98625-26-4.
Activity(S)(-)-BayK8644isanL-typeCa2+-channelactivator. Itdisplaysvasoconstrictiveeffects invivo1.
- Franckowiak,G. etal. (1985) Eur.J.Pharmacol. 114, 223.
ShippingandstorageShippedatroomtemperature.Productassuppliedcanbestoredintactatroomtemperatureforseveralweeks.Forlongerperiods,itshouldbestoredat-20°C.
Solubility100mMinDMSO.Centrifugeallproductpreparationsbeforeuse(10000xgfor1min).
StorageofsolutionsUptofourweeksat4°Corthreemonthsat-20°C.
AlomoneLabs(S)(-)-BayK8644increasesL-typeCaV channelcurrentsheterologouslyexpressedin Xenopus oocytes.A.TimecourseofCaV1.2(co-expressedwithα2δ1andβ1auxiliarysubunits)peakcurrentamplitude,elicitedby100msvoltagerampfromholdingpotentialof-100mVto+50mV,deliveredevery10seconds.Applicationof0.1and1µM (S)(-)-BayK8644 (#B-351)increasestheCaV1.2current(indicatedbythehorizontalbar).B.Representativecurrenttracesbeforeandduringapplicationof0.1and1µM(S)(-)-BayK8644asindicated.
References-Scientificbackground
- 1.Hadley,R.W.andHume,J.R. (1988) Circ.Res. 62, 97.
- 2.Wei,X.Y. etal. (1986) J.Pharmacol.Exp.Ther. 239, 144.
- (S)(-)-BayK8644isanL-typeCa2+-channelactivatorwithpositiveinotropic,vasoconstrictiveandbehavioraleffectsinvivo.Itisanenantiomerof(±)-BayK8644.(S)(-)-BayK8644isadihydropyridinethatcan,amongothereffects,constrictbloodvesselsandincreasetheforceofcontractionoftheheart.ThedrugisthoughttoincreasetheentryofCa2+intocellsbyincreasingtheopen-stateprobABIlityofCa2+channels,eitherbyprolongingthedurationofindividualopeningsorbyincreasingthenumberofopenings.ItwasnotedinearlyexperimentswithBayK8644thatthedose-responsecurveisbiphasic1.Theactionof(S)(-)-BayK8644wasstudiedinrattailarteryandguineapigileallongitudinalsmoothmuscleusingpharmacologicandrADIoligandbindingassays.Itinducedconcentration-dependentcontractionsinbothsmoothmusclepreparationswithEC50valuesof1.98-11nM.Withincreasingconcentrations,(S)(-)-BayK8644relaxedthetissuewithIC50valuesof0.53-2.60μM2.
TargetL-typeCa2+ channels
LyophilizedPowder
Clickheretoreceivea1mgfreetrialsample!
Lastupdate:24/09/2019
(S)(-)-BayK8644(#B-351) isahighlypure,synthetic,andbiologicallyactivecompound.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
