Catalogue numbercrb2005114AntibodyAnti-B-Raf antibodyAntigen PeptideKLH conjugated synthetic peptide crb1200381eProtein IDUniProtKB - P15056AliasesSerine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, BRAF, RAFB1, P94, V-Raf Murine Sarcoma Viral Oncogene Homolog B Cross-ReactivityHuman, rat, mouse, chickenHost Species RabbitAntibody TypePolyclonalConcentration0.1mg/mlValidation1:1000 (WB), 1:1000 (ELISA)TargetB-RafFamilyTKL Ser/Thr protein kinase family. RAF subfamily.Super FamilyProtein kinase superfamilyStorage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. References

Eisenhardt, A., Sprenger, A., Röring, M., Herr, R., Weinberg, F., Köhler, M., Braun, S., Orth, J., Diedrich, B., Lanner, U., Tscherwinski, N., Tscherwinski, S., Schuster, S., Dumaz, N., Schmidt, E., Baumeister, R., Schlosser, A., Dengjel, J. and Brummer, T. (2016). Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles. Oncotarget, 7(18). PMID: 27034005

Li, Y., Takahashi, M. and Stork, P. (2013). Ras-mutant Cancer Cells Display B-Raf Binding to Ras That Activates Extracellular Signal-regulated Kinase and Is Inhibited by Protein Kinase A Phosphorylation. J Biol Chem, 288(38), 27646-27657. PMID: 23893412

Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。