Analysis of Differentially Expressed Proteome in Urinary Exosome from Non-small Cell lung Cancer Patients DAI Yi1,ZHANG Yuan1,QIU Feng2,LI Yan-Yan1,QIU Zong-Yin* 1 1 ( College of Pharmacy,Chongqing Medical University,Chongqing 400016) 2 ( First Affiliated Hospital,Chongqing Medical University,Chongqing 400016)Urine provides an alternative to blood plasma as a potential source of disease biomarkers. Exosomes was separated by ultracentrifuge at 200000 g in normal persons and non-small cell lung cancer ( NSCLC) patients′ urine. For proteomic analysis of urinary exosome,1D sodium dodecylsulfonate-polyacrylate gel electrophoresis( SDS-PAGE) was carries out and cut the gel 31 kDa-20 kDa bands in normal group and disease group′s. These gel blocks were subjected to in-gel trypsinization,and the extracted peptides were analyzed HPLC-CHIP-MS/MS. Approximately 24 unique proteins were identified in the UniProtKB/SWISSPORT. The difference expression proteins were found in urinary exosome from NSCLC patients,including three fragment of the immunoglobulin kappa,two kinds of Ras related proteins,glutathione S-transferase A2, serum amyloid P-component precursor and phosphatidylethanolamine-binding protein 1. 【CateGory Index】： R734.2 Download(CAJ format) Download(PDF format) CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format. LI Yan-yan,ZHANG Yuan,DAI Yi,QIU Feng,QIN Xia,QIU Zong-yin(College of Pharmacy,Chongqing Medical University,Chongqing 400016,China);Isolation and Identification of Exosomes in Human Urine[J];Chinese Journal of Biologicals;2011-02 DAI SongWei1,2, WANG XiaoMin1,2, LIU LiYun1,2, LIU JiFu3, WU ShanShan3, HUANG LingYun1,2, XIAO XueYuan1,2 HE DaCheng1,2 1 Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Beijing Normal University, Beijing 100875, China; 2 Universities’ Confederated Institute of Proteomics, Beijing 100875, China; 3 Department of Thoracic Surgery, General Hospital of Beijing Unit, PLA, Beijing 100500, China;Discovery and identification of Serum Amyloid A protein elevated in lung cancer serum[J];中国科学(C辑:生命科学)(英文版);2007-03 LIU Jun-lian,LI Yong-zhi,GAO Jian-yi,GAI Yu-qing,WANG Jing,XUE Chun-mei,XIN Bing-mu.China Astronaut Research and Training Center,Beijing 100193,China;The Progress of the Proteomic Technology.[J];Space Medicine Medical Engineering;2009-02 DENG Qing-nan,ZHOU Jian-long,ZHANG Ping(General Hospital of Guangzhou Military Area Command,Guangzhou 510010,China);SELDI Technique and Its Application in Lung-related Diseases Research[J];Chinese Medical Equipment Journal;2009-04 GE Chun-lin~1 LUAN Feng-ming~1 MA Ning~1 LI Yong-zhe~2 HU Chao-jun~2 LI Ning~2 1 Department of General Surgery,First Affiliated Hospital,China Medical University,Shenyang llO001,China; 2 Department of Clinical Laboratory,Peking Union Medical College Hospital,Peking Union Medical College Chinese Academy of Medical Sciences,Beijing 100730,China;Diagnosis of Tumor-Like Polypoid Lesions of Gallbladder by Serum Proteomic Fingerprint[J];中国癌症研究(英文版);2008-04 ZHOU Yan-hong,CAO Jia,CHEN Jun,CAI Zhi-min,WANG Zi-qiang (Department of Hygien Toxicology,College of Preventive Medicine,Third Military Medical University,Chongqing 400038,China);Isolation and determination of exosomes from ascites of patients with advanced gastric cancer[J];Immunological Journal;2008-06 LI Qi-ling~ 1,2 , BU Ning~2, LI Ya-ling~3, YU Yue-cheng~2, LU Qin-rui~1, XIN Xiao-yan~21.Department of Gynecology and Obstetrics, First Affiliated Hospital, Xi\'an Jiaotong University, Xi\'an 710061,Shannxi Province, P.R.China2.Department of Haematology, Xijing Hospital, Fourth Military Medical University, Xi\'an 710032, ShannxiProvince, P.R. China3.Gongzhuling Health School, Gongzhuling 136100, Jilin Province, P.R.China;Separation and Identification of exosomes origined from ascites[J];Chinese Journal of Cancer Prevention and Treatment;2006-20 ZHU Lei, LI Xiang, LIU Peng-fei, et al(Bioengineering Experimental Center, College of Pharmacology, Jilin University, Changchun 130021, China);Progress in Research on Role of Exosomes in Tumor Immunity[J];Chinese Journal of Biologicals;2010-04 LIU Deng-qun1,WANG Feng-chao1,TAO Zhong-fen2,DONG Shi-wu1,ZOU Zhong-min1,WANG Jun-ping1,RAN Xin-ze1,SU Yong-ping1(1State Key Laboratory of Trauma,Burn and Combined Injury,Institute of Combined Injury,College of Preventive Medicine,Third Military Medical University,Chongqing 400038,China;2Dept of Center Laboratory,College of Medicine,Third Military Medical University);Immunoelectromicroscopy observation of donor-derived cells in intestinal epithelium of recipient mice after bone marrow transplantation[J];Journal of Shanxi Medical University;2009-02 MA Ren-fu,HOHG Ni,XIAO Jian(Health and Nursing Department,Wuhan Polytechnic University,Wuhan 430023,China);Sepation and Identification of exosomes from Malignant Pleural Effusion[J];Journal of Wuhan Polytechnic University;2008-02

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