Background:PRMT8 is a histone methyltransferase that exhibits methylation activity toward histones H2A and H4. Description:The PRMT8 Homogeneous Assay Kit is designed to measure the activity of PRMT8 for screening and profiling applications, using AlphaLISA^® technology. The key to the PRMT8 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT8 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Supplied As: The PRMT8 Homogeneous Assay Kit comes in a convenient AlphaLISA^® format, with a 384-well plate, biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT8 for 384 enzyme reactions. Contraindications: Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays. Format: Instructions for use: See assay kit data sheet for detailed protocol. Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Reference(s): Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Methyltransferase Product Type: Assay Kit Data shown is lot-specific. Contact us for specific information on other lots.

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